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商品描述
| CatalogNumber: 10116 | |||||||||
| ProductName: Rab5Protein | |||||||||
| Synonyms: MemberRASoncogenefamily,RAB5A | |||||||||
| Source: Human,recombinantfulllength,His6-tag | |||||||||
| ExpressionHost: E.coli | |||||||||
| MolecularWeight: 17kDa | |||||||||
| Purity: >95%bySDS-PAGE | |||||||||
| Introduction: Rab5,consistsofthreeisoforms,Rab5A,Rab5B,andRab5C,isasmallGTPasethatislocalizedtoearlyendosomes.Itregulatesthefusionbetweenendocyticvesiclesandearlyendosomes,aswellasthehomotypicfusionbetweenearlyendosomes. | |||||||||
| AminoAcidSequence (16-184) | |||||||||
NKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERY HSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQ SYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKN| Properties | PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. | PhysicalAppearance(form): Whiteorclear | Concentration: 1mg/mL | Storage: -80°C | PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedRab5wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining | References: 1.Bucci,C.etal.,Cell70:715-728,1992.2.Kinchen,J.M.etal.,Nature464:778-782,2010.3.Kitano,M.etal.,Nature453:241-245,2008.4.Lanzetti,L.etal.,Nature429:309-314,2004.5.Lanzetti,L.etal.,Nature408:374-377,2000.6.Miaczynska,M.etal.,Cell116:445-456,2004.7.Ohya,T.etal.,Nature459:1091-1097,2009.8.Otomo,A.etal.,Hum.Molec.Genet.12:1671-1687,2003.9.Rousseau-Merck,M.F.etal.,Hum.Genet.86:350-354,1991.10.Stenmark,H.etal.,Cell83:423-432,1995.11.Xiao,G.-H.etal.,J.Biol.Chem.272:6097-6100,1997.12.Zahraoui,A.etal.,J.Biol.Chem.264:12394-12401,1989. | Datasheet: |  |
