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商品描述
| CatalogNumber: 10138 | ||||||||||||||
| ProductName: Gα13Protein | ||||||||||||||
| Synonyms: Guaninenucleotide-bindingproteinsubunitalpha-13,Galpha13,G13 | ||||||||||||||
| Source: Human,recombinantfulllength,His6-tag | ||||||||||||||
| ExpressionHost: sf9cells | ||||||||||||||
| MolecularWeight: 44kDa | ||||||||||||||
| Purity: >95%bySDS-PAGE | ||||||||||||||
| Introduction: Ga13belongstotheG12familyofheterotrimericguaninenucleotide-bindingproteins,andhasintrinsicGTPaseactivity.Ga13hasbeenrevealedtoplaycriticalrolesintransformation,normalhemostasisandthrombosis,growthfactor-inducedcellmigration,angiogenesis,andsalt-inducedhypertension. | ||||||||||||||
| AminoAcidSequence (1-377) | ||||||||||||||
MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQ MRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQ GMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTK GIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ| Properties | PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. | PhysicalAppearance(form): Whiteorclear | Concentration: 1mg/mL | Storage: -80°C | PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedGα13wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining. | References: 1.Gong,H.etal.,Science327:340-343,2010.2.Kabouridis,P.S.etal.,Molec.Cell.Biochem.144:45-51,1995.3.Kilts,J.D.etal.,J.Cardiovasc.Pharm.50:299-303,2007.4.Moers,A.etal.,NatureMed.9:1418-1422,2003.5.Offermanns,S.etal,Science275:533-536,1997.6.Radhika,V.etal.,J.Biol.Chem.279:49406-49413,2004.7.Ruppel,K.M.etal.,Proc.Nat.Acad.Sci.102:8281-8286,2005.8.Shan,D.etal.,Dev.Cell10:707-718,2006.9.Wirth,A.etal.,NatureMed.14:64-68,2008. | Datasheet: | 
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