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Introduction: SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs).

AminoAcidSequence (1-191)

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL RPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQ KPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Properties

PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.

PhysicalAppearance(form): Whiteorclear

Concentration: 1mg/mL

Storage: -80°C

PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedCdc42wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining.

References: 1.Garrett,W.S..etal.,Cell102:325-334,2000.2.Irie,F.etal.,NatureNeurosci.5:1117-1118,2002.3.Kawasaki,Y.etal.,Oncogene26:7620-7627,2007.4.Manser,E.etal.,Nature363:364-367,1993.5.Musch,A.etal.,EMBOJ.20:2171-2179,2001.6.Nalbant,P.etal.,Science305:1615-1619,2004.7.Shen,Y.etal.,Dev.Cell14:342-353,2008.8.Wu,W.J.etal.,Nature405:800-804,2000.9.Wu,X.etal.,GenesDev.20:571-585,2006.10.Zheng,Y.etal.,J.Biol.Chem.271:33169-33172,1996.

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