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商品描述
| Catalog Number: 26287 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti-EGFR (L747-P753) S Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR (L747-P753) S, but not wild type EGFR protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Download Datasheet:  |
| Western blot: |
| | Western blot analysis of recombinant EGFR(L747-P753) S and wildtype proteins. Purified His-tagged EGFR (L747-P753) S protein (702-987) and wild-type protein (702-987) were blotted with anti- EGFR (L747-P753) S mouse antibody (Cat. #26287). |
| Immunofluorescence: |
 | Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (L747-P753) S antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (L747-P753) S plasmid (right column), then fixed and stained with anti- EGFR (L747-P753) S monoclonal antibody (Cat. #26287). |
| Related Products | |||||
| Catalog# | Name | Size | Price | DataSheet | |
| 26287 | Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody | 100 μL | $495 |  | |
| 26119 | Anti-EGFR(G863D) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26083 | Anti-EGFR(G719S) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26082 | Anti-EGFR (L858R) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26077 | Anti-EGFR (L861Q) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26115 | Anti-EGFR(G719A) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26120 | Anti-EGFR(D761Y) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26121 | Anti-EGFR(V769L) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26117 | Anti-EGFR(A839T) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26116 | Anti-EGFR(N826S) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26176 | Anti-EGFR (vIII) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26221 | Anti-EGFR (L858Q) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26481 | Anti-EGFR (T854A) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| 26483 | Anti-EGFR (S768I) Mouse Monoclonal Antibody | 100 μL | $495 | | |
| Publications: |
| 1. Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma Tumor Biology. October 2014 |
