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商品描述
| CatalogNumber: 26084 |
| GeneSymbol: IDH2 |
| Description: Anti-IDH2(R172H)MouseMonoclonalAntibody |
| Background: Isocitratedehydrogenase(IDH)catalyzestheoxidativedecarboxylationofisocitrateto2-oxoglutarate.MutationsofArg-172inIDH2toCys,His,LeuorSerabolishmagnesiumbindingandtheconversionofisocitratetoalpha-ketoglutarate.Instead,alpha-ketoglutarateisconvertedtoR-2-hydroxyglutarate.ElevatedlevelsofR-2-hydroxyglutaratearecorrelatedwithanelevatedriskofmalignantbraintumors. |
| Immunogen: AsyntheticpeptidefromtheinternalregionofhumanIDH2whichincludesthemutationofR172H. |
| Applications: ELISA,WB,IHC,IF |
| RecommendedDilutions: ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:100IF:1:50-1:100 |
| Concentration: 1mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purifiedfromascites |
| Storagebuffer: |
| Preservative: no |
| Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
| SpeciesReactivity: Anti-IDH2(R172H)antibodyrecognizesIDH2(R172H)ofvertebrates. |
| StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles. |
DownloadDatasheet:  |
| Westernblot: |
| WesternblotanalysisofrecombinantIDH2(R172H)andwild-typeIDH2proteins.Purifiedhis-taggedIDH2(R172H)protein(Cat.#10201)andwild-typeIDH2protein(Cat.#10200)wereblottedwithanti-IDH2(R172H)monoclonalantibody(Cat.#26084)(leftpanel).ProteinsloADIngwereblottedwithanti-IDH2monoclonalantibody(rightpanel). |
| Immunofluorescence: |
 | ImmunofluorescenceofcellsexpressingIDH2proteinswithanti-IDH2(R172H)antibody.HEK293TcellsweretransfectedwithpCDNA3-GFP-IDH2(WT)plasmid(leftcolumn)orpCDNA3-GFP-IDH2(R172H)plasmid(rightcolumn),thenfixedandstainedwithanti-IDH2(R172H)monoclonalantibody(Cat.#26084). |
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| 26084 | Anti-IDH2(R172H)MouseMonoclonalAntibody | 100μL | $495 | | |
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| 26164 | Anti-IDH2(R172W)MouseMonoclonalAntibody | 100μL | $495 | | |
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Publications:| 1. SpecificmonoclonalantibodiesagainstIDH1/2mutationsasdiagnostictoolsforgliomas BrainTumorPathology.January2015,Volume32,Issue1,pp3-11 | 2. MutantIDHinhibitsHNF-4αtoblockhepatocytedifferentiationandpromotebiliarycancer Nature513,110–114(04September2014) | 3. Metabolomiccomparisonbetweencellsoverexpressingisocitratedehydrogenase1and2mutantsandtheeffectsofaninhibitoronthemetabolism JournalofNeuRochemistry.Volume132,Issue2,pages183–193,January2015 | |
