NewEast/EYA2 [26052][100 µL]/26052

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¥4780.00
货号:26052
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品牌:NewEastBio
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商品描述

Catalog Number:  26052
Gene Symbol:  EYA2
Description:   Anti- EYA2 Mouse Monoclonal Antibody
Background:   EYA2 (eyes absent homolog 2)functions as a tyrosine phosphatase that specificallydephosphorylates Tyr-142 of histone H2AX. EYA2 promotes efficientDNA repair and coactivates SIX1. Together with SIX1 and DACH2, EYA2seems to be involved in myogenesis.
Immunogen:   A synthetic peptide near theN-terminus of human EYA2.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-EYA2 antibody recognizesEYA2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze /thaw cycles.
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant full-length EYA2 protein.Purified His-tagged EYA2 protein (Cat. # 10195) was blotted withanti-EYA2 monoclonal antibody (Cat. # 26052).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded humanadenocarcinoma tissue with anti-EYA2 monoclonal antibody (Cat. #26052). Tissue section was fixed with formaldehyde and blocked with1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediationin citrate buffer (pH6). Samples were then incubated with primaryantibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouseIgG (dilution 1:50) was used as secondary antibody.
 
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