NewEast/Gαi [21006][100 µL]/21006

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¥4380.00
货号:21006
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品牌:NewEastBio
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商品描述

CatalogNumber:  21006
GeneSymbol:  i
Description:  Anti-GαiRabbitPolyclonalAntibody
Background:  HeterotrimericGproteinsarecriticalcellularsignaltransducers.Gαirepresentsonesub-familyofGproteinsthatcouldmediatetheinhibitionofadenylylcyclases.OtherbiochemicalandphysiologicalfunctionsofGαiproteinsarebeingexplored.
Immunogen:  RecombinantfulllengthproteinofGαi1
Applications:  ELISA,WB,IHC
RecommendedDilutions:  ELISA1:100-1:5000,WB1:100-1:2000
Concentration:  1mg/ml
Host:  Rabbit
Format:  Liquid
Clonality:  Polyclonal
Isotype:  
Purity:  Purifiedfromserum
Storagebuffer:
Preservative:  no
Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol
SpeciesReactivity:  iantibodyrecognizesGαi1,Gαi2,andGαi3ofvertebrates
StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles
DownloadDatasheet:  
Westernblot:  
WesternblotanalysisofGiproteins.Gαi1plasmidDNAwastransientlytransfectedintoHEK-293cells.Whole-celllysatewasusedforwesternblotwiththepolyclonalanti-Gαiantibody(cat#21006).
 
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Publications:
1.  IdentificationofnovelsignallingrolesandtargetsforGalphaandGbetagammadownstreamoftheinsulin-likegrowthfactor1receptorinvascularsmoothmusclecells    BiochemJ.2013Feb15;450(1):209-19
2.  CRHactivationofdifferentsignalingpathwaysresultsindifferentialcalciumsignalinginhumanpregnantmyometriumbeforeandduringlabor    JClinEndocrinolMetab.2012Oct;97(10):E1851-61
3.  CXCR4activationdefinesanewsubgroupofSonichedgehog-drivenmedulloblastoma    CancerRes.2012Jan1;72(1):122-32
4.  RegulationofestrADIolandprogesteroneproductionbyCRH-R1and-R2isthroughdivergentsignalingpathwaysinculturedhumanplacentaltrophoblasts    Endocrinology.2012Oct;153(10):4918-28
5.  WNT-5AstimulatestheGDP/GTPexchangeatpertussistoxin-sensitiveheterotrimericGproteins    CellSignal.2011Mar;23(3):550-4
6.  HeterotrimericGalpha(i)proteinsareregulatedbylipopolysaccharideandareanti-inflammatoryinendotoxemiaandpolymicrobialsepsis    BiochimBiophysActa.2011Mar;1813(3):466-72
7.  Estrogen-andxenoestrogen-inducedERKsignalinginpituitarytumorcellsinvolvesestrogenreceptor-αinteractionswithGprotein-αiandcaveolinI    SteroidsVolume77,Issue5,April2012,Pages424–432
8.  Na/HexchangerregulatoryfactorscontrolparathyroidhormonereceptorsignalingbyfacilitatingdifferentialactivationofG(alpha)proteinsubunits    JBiolChem.2010Aug27;285(35):26976-86
9.  Beta-arrestin-butnotGprotein-mediatedsignalingbythe"decoy"receptorCXCR7    ProcNatlAcadSciUSA.2010Jan12;107(2):628-32
10.  StructuralbasisforactivationoftrimericGiproteinsbymultiplegrowthfactorreceptorsviaGIV/Girdin    MolBiolCell.2014Nov5;25(22):3654-71
11.  CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamicstoPromoteMacrophagePhagocytosisofZymosan    MolCellBiol.2014Nov15;34(22):4186-99
12.  FattyAcid-bindingProtein5(FABP5)RegulatesCognitiveFunctionBothbyDecreasingAnandamideLevelsandbyActivatingtheNuclearReceptorPeroxisomeProliferator-activatedReceptorβ/δ(PPARβ/δ)intheBrainJBiolChem.2014May2;289(18):12748-58