ProductHighlight

• Configuration-specificmonoclonalantibodythatrecognizesRap1-GTP,butnotRap1-GDP
• TheonlyavailableRap1activationassaykitdirectlymeasuresthelevelofRap1-GTP
• Providingshorterexperimentaltime
• CouldbeusedtomonitortheactivationofRap1incellsandintissuesbyimmunohistochemistry

ProductDescription

SmallGTPasesareasuper-familyofcellularsignalingregulators.ThesmallRas-likeGTPaseRap1isanevolutionaryconservedproteinthatoriginallygainedinterestbecauseofitscapacitytorevertthemorphologicalphenotypeofRas-transformedfibroblasts.Rap1isregulatedbyalargenumberofstimulithatincludegrowthfactorsandcytokines,butalsophysicalforceandosmoticstress.Rap1wasshowntoregulatemultiplebasiccellularprocesses.ThebeststudiedaspectofRap1functioninendothelialcellsinvolveditsroleinregulationofcell-celljunctionformationandremodeling.

CurrentlythereisnodirectassaytomeasuretheactivationofRap1GTPases.

NewEastBiosciencesRap1ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRap1-GTP,butnotRap1-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Theseanti-Rap1-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofRap1incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesRap1ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRap1.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesRap1ActivationAssayKitbasesontheconfiguration-specificanti-Rap1-GTPmonoclonalantibodytomeasuretheactiveRap1-GTPlevels,eitherfromcellextractsorfrominvitroGTPΓSloADIngRap1activationassays.Briefly,anti-activeRap1mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRap1-GTP.TheboundactiveRap1willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRap1willbedetectedbyimmunoblotanalysisusinganti-Rap1rabbitpolyclonalantibody.

   ProductManual
   ProtocolforIF/IHC

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
26912	ActiveRap1-GTPMonoclonalAntibody	30μL	$495
81401	Rap1ActivationAssayKit	20assays	$637

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Publications:

1.  Rap1GTPaseActivationandBarrierEnhancementinRPEInhibitsChoroidalNeovascularizationInVivo    PLoSOne.2013Sep10;8(9):e73070