ProductHighlight

• Configuration-specificmonoclonalantibodythatrecognizesCdc42-GTP,butnotCdc42-GDP
• TheonlyavailableCdc42activationassaykitdirectlymeasuresthelevelofCdc42-GTP
• ProvidingreliableresultswithconsistentreproducibilitythanotherCdc42activationassaykitsbasedoncertaindownstreamsignalingpathways
• Providingshorterexperimentaltime
• CouldbeusedtomonitortheactivationofCdc42incellsandintissuesbyimmunohistochemistry

ProductDescription

SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.

CurrentlytheactivationofCdc42proteinsisassayedwiththebindingofGTP-boundCdc42tothep21-bindingdomain(PBD)ofp21-activatedproteinkinase(PAK).Thismethodisbasedontheobservationthattheactive,GTP-boundCdc42(andRac)couldbindtothePBDofPAK.However,thereproducibilityofthismethodispoor.ThisispartiallyduetotherelativelyquickhydrolysisofGTPtoGDPduringtheassayprocedure,andthelowbindingaffinityofPBDtoCdc42-GTP.

NewEastBiosciencesCdc42ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesCdc42-GTP,butnotCdc42-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinamuchshortertime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Theseanti-Cdc42-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofCdc42incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesCdc42ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofCdc42.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesCdc42ActivationAssayKitbasesontheconfiguration-specificanti-Cdc42-GTPmonoclonalantibodytomeasuretheactiveCdc42-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngCdc42activationassays.Briefly,anti-activeCdc42mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingCdc42-GTP.TheboundactiveCdc42willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveCdc42willbedetectedbyimmunoblotanalysisusinganti-Cdc42rabbitpolyclonalantibody.

   ProductManual
   ProtocolforIF/IHC

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
80701	Cdc42ActivationAssayKit	20assays	$637
26905	ActiveCdc42-GTPMonoclonalAntibody	30μL	$495
26008	Anti-Cdc42MouseMonoclonalAntibody	100μL	$275
21010	Anti-Cdc42RabbitPolyclonalAntibody	100μL	$275

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Chemicell产品 | 转染试剂24765 | percipiobio价格 | Probetex价格 | bioroute华东代理 | Glixx Laboratories抗体 | Sigma代理 | 蚂蚁淘 | 染色体倍性分析仪

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Publications:

1.  VibrioparahaemolyticusEffectorProteinsSuppressInflammasomeActivationbyInterferingwithHostAutophagySignaling    PLoSPathog.2013Jan;9(1):e1003142

2.  Interferongammainducesactinpolymerization,Rac1activationanddownregulatesphagocytosisinhumanmonocyticcells    Cytokine.2012Jan;57(1):158-68

3.  Invadopodiaandrolling-typemotilityarespecificfeaturesofhighlyinvasivep190bcr-ablleukemiccells    EuropeanJournalofCellBIOLOGyVolume91,Issues11–12,November–December2012,Pages978–987

4.  EpithelialjunctionformationrequiresconfinementofCdc42activitybyanovelSH3BP1complex    JCellBiol.2012Aug20;198(4):677-93

5.  PolarizedmigrationoflymphaticendothelialcellsiscriticallydependentonpodoplaninregulationofCdc42    AmJPhysiolLungCellMolPhysiol.2011Jan;300(1):L32-42

6.  TheAarskog-ScottSyndromeProteinFgd1RegulatesPodosomeFormationandExtracellularMatrixRemodelinginTransformingGrowthFactorbeta-StimulatedAorticEndothelialCells    MolCellBiol.2011Nov;31(22):4430-41

7.  MMP-9andCXCL8/IL-8ArePotentialTherapeuticTargetsinEpidermolysisBullosaSimplex    PLoSOne.2013Jul19;8(7):e70123

8.  p21-ActivatedKinase(PAK)RegulatesCytoskeletalReorganizationandDirectionalMigrationinHumanNeutrophils    PLoSOne.2013Sep3;8(9):e73063

9.  TheCdc42GuanineNucleotideExchangeFactorFGD6CoordinatesCellPolarityandEndosomalMembraneRecyclinginOsteoclasts    JBiolChem.2014Jun27;289(26):18347-59

10.  Cdc42InhibitorandUsesThereof    UnitedStatesPatentApplication20140194451

11.  Theα4NicotinicReceptorPromotesCD4+T-CellProliferationandaHelperT-CellImmuneResponse    MolecularPharmacologyJanuary2014vol.85no.150-61