ProductHighlight

• Configuration-specificmonoclonalantibodythatrecognizesArl1-GTP,butnotArl1-GDP
• TheonlyavailableArl1activationassaykitdirectlymeasuresthelevelofArl1-GTP
• Providingshorterexperimentaltime
• CouldbeusedtomonitortheactivationofArl1incellsandintissuesbyimmunohistochemistry

ProductDescription

Arf-likeprotein1(Arl1)isamemberoftheArffamilyofregulatoryGTPases,withintheRassuperfamilyofGTPases,andwithhighlyconservedorthologsthroughouteukaryotes.Arl1isessentialforearlyembryonicdevelopmentinDrosophilaandinCaenorhaBDitiselegans.Arl1ismostsimilarinprimarysequence,cellularlocation,andfunction(regulationofmembranetraffic)toArf1-6andevensharesseveralcommonbindingpartners.InadditiontoitsfunctioninmembranetrafficattheGolgi/trans-Golginetwork,therearereportsindicatingapossIBLeroleforArl1inionhomeostasisinyeast.

CurrentlythereisnodirectassaytomeasuretheactivationofArl1GTPases.

NewEastBiosciencesArl1ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesArl1-GTP,butnotArl1-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Theseanti-Arl1-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofArl1incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesArl1ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofArl1.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesArl1ActivationAssayKitbasesontheconfiguration-specificanti-Arl1-GTPmonoclonalantibodytomeasuretheactiveArl1-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngArl1activationassays.Briefly,anti-activeArl1mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingArl1-GTP.TheboundactiveArl1willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveArl1willbedetectedbyimmunoblotanalysisusinganti-Arl1rabbitpolyclonalantibodyoranti-Arl1mousemonoclonalantibody.

   ProductManual

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
26924	ActiveArl1-GTPMonoclonalAntibody	30μL	$495
82901	Arl1ActivationAssayKit	20assays	$637
26056	Anti-Arl1MouseMonoclonalAntibody	100μL	$239

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