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蚂蚁淘在线 / 品牌中心 / NewEastBio / NewEast/Arl3 Activation Assay Kit [83001][20 Assays]/83001

NewEast/Arl3 Activation Assay Kit [83001][20 Assays]/83001

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￥14740.00

货号：83001

浏览量：102

品牌：NewEastBio

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商品描述

ProductHighlight

Configuration-specificmonoclonalantibodythatrecognizesArl3-GTP,butnotArl3-GDP
TheonlyavailableArl3activationassaykitdirectlymeasuresthelevelofArl3-GTP
Providingshorterexperimentaltime
CouldbeusedtomonitortheactivationofArl3incellsandintissuesbyimmunohistochemistry

ProductDescription

Arl3(Arf-like3)isanADP-ribosylationfactors(Arf)familyprotein.Arl3interactswithandregulatesactivitiesofseveraleffectorproteins,suchashumanretinalgene4(HRG4),δ-subunitofthecGMPphosphodiesterase(PDEδ),andbinderofArl2(BART).Arl3alsobindsmicrotubulestoalterspecificaspectsofcytokinesisviainteractionswithretinitispigmentosa2(RP2).

CurrentlythereisnodirectassaytomeasuretheactivationofArl3GTPases.

NewEastBiosciencesArl3ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesArl3-GTP,butnotArl3-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Theseanti-Arl3-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofArl3incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesArl3ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofArl3.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesArl3ActivationAssayKitbasesontheconfiguration-specificanti-Arl3-GTPmonoclonalantibodytomeasuretheactiveArl3-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngArl3activationassays.Briefly,anti-activeArl3mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingArl3-GTP.TheboundactiveArl3willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveArl3willbedetectedbyimmunoblotanalysisusinganti-Arl3rabbitpolyclonalantibody.


RelatedProducts
Catalog#	Name	Size	Price	DataSheet
26925	ActiveArl3-GTPMonoclonalAntibody	30μL	$495
83001	Arl3ActivationAssayKit	20assays	$637
26070	Anti-Arl3MouseMonoclonalAntibody	100μL	$239

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