ProductHighlight

• Configuration-specificmonoclonalantibodythatrecognizesRab5-GTP,butnotRab5-GDP
• TheonlyavailableRab5AactivationassaykitdirectlymeasuresthelevelofRab5A-GTP
• Providingshorterexperimentaltime
• CouldbeusedtomonitortheactivationofRab5incellsandintissuesbyimmunohistochemistry

ProductDescription

SmallGTPasesareasuper-familyofcellularsignalingregulators.Rab5isamemberoftheRab-familyGTPases.Rab5isarate-limitingcomponentofthemachineryregulatingthekineticsofmembranetrafficintheearlyendocyticpathway.

CurrentlythereisnodirectassaytomeasuretheactivationofRabGTPases.

NewEastBiosciencesRab5AActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab5-GTP,butnotRab5-GDP,andaRab5Aspecificrabbitpolyclonalantibody.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Thisanti-Rab5-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofRab5incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesRab5AActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab5A.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesRab5AActivationAssayKitbasesontheconfiguration-specificanti-Rab5-GTPmonoclonalantibodytomeasuretheactiveRab5A-GTPlevels,eitherfromcellextractsorfrominvitroGTP_γSloADIngRab5Aactivationassays.Briefly,anti-activeRab5mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab5-GTP.TheboundactiveRab5willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab5Awillbedetectedbyimmunoblotanalysisusinganti-Rab5Aspecificrabbitpolyclonalantibody.

   ProductManual

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
83701	Rab5ActivationAssayKit	20assays	$637
81301	Rab5AActivationAssayKit	20assays	$637
83401	Rab5BActivationAssayKit	20assays	$637
83501	Rab5CActivationAssayKit	20assays	$637
26911	ActiveRab-GTPMonoclonalAntibody	30μL	$495
26016	Anti-Rab5MouseMonoclonalAntibody	100μL	$239
21151	Anti-Rab5RabbitPolyclonalAntibody	100μL	$219
21036	Anti-Rab5ARabbitPolyclonalAntibody	100μL	$219
21037	Anti-Rab5BRabbitPolyclonalAntibody	100μL	$219
21038	Anti-Rab5CRabbitPolyclonalAntibody	100μL	$219

NewEastBio我们的理念是Our Vision以信誉求发展，以质量树品牌，为科研服务，为生命尽责

• 

  资质认证

  获得国家资质，权威认证！

• 

  全国联保

  全国联保，官方无忧售后

• 

  正规发票

  正规发票，放心购买

• 

  签订合同

  签订合同，保障您的权益

购物指南

购物流程

注册登录

账户管理

订单查看

常见问题

FAQS

蚁豆兑换

物流说明

评论规则

发票说明

价格说明

支付说明

服务协议

版权声明

诚信保障

免责申明

销售协议

隐私条款

法律申明

售后服务

退换货政策

工作时间

退款说明

售后支持

售后申请表模板列表

关于我们

公司简介

联系我们

资质证书

人才招聘

商务合作

万众创业

网址导航

蚂蚁淘商城

科研狗

苏州蚂蚁淘

生物信息网

现货促销


微信公众号

biopioneerinc中国 | Polysciences阳离子聚合物基因 | nanocomposix价格 | NSJ Bio说明书 | Benifuji华东代理 | fitzgerald-fii | proliantbiologicals授 | 蚂蚁淘 | 护手护脚用品

copyright©2005-2021 蚂蚁淘在线版权所有　苏州蚂蚁淘生物科技有限公司 www.assay-online.com ICP号：苏ICP备17049038号-16

/**/

Publications:

1.  VASPpromotesTGF-βactivationofhepaticstellatecellsbyregulatingRab11dependentplasmamembranetargetingofTGF-βreceptors    HepatologyVolume61,Issue1,pages361–374,January2015

2.  Rab5ActivityRegulatesGLUT4SortingintoInsulin-ResponsiveandNon-Insulin-ResponsiveEndosomalCompartments:APotentialMechanismforDevelopmentofInsulinResistance    Endocrinology.2014Sep;155(9):3315-28