Description
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Liver In Vivo Transfection Reagent
siRNA and DNA liver in vivo delivery reagent for animal research (mouse, rat)
Modes of administration:
- Systemic intravenous (i.v.) injection
- Direct intratumoral (i.t.) injection
- Intraperitoneal (i.p.) injection
Liver-targeted In Vivo Transfection Reagent
- Biodegradable lipid liposome-based reagent
- Liposome conjugated complexes are stable in serum (16h)
- Efficient delivery to the liver tissue (via systemic administration) and liver tumors (via intratumoral administration)
- Efficient siRNA, shRNA, microRNA, and plasmid DNA delivery
- Minimal toxicity
- Functionally validated in mice
- Applicable for plasmid DNA/siRNA co-injection
- Download Liver-targeted in vivo transfection protocol: [PDF] [Word]
- Download PowerPoint presentation for Liver-targeted in vivo transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
DATA
Figure 1. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).
Figure 2. Intratumoral administration (i.t.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (liver cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).
Figure 3. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.
Selected Liver InVivo Transfection Reagent citation references:
- Molecular Therapy Nucleic Acids. 2017. Reverse Expression of Aging-Associated Molecules through Transfection … Kim H. et al [PDF]
- PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF]
- Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
- Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
- J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]
- J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]
Liver In Vivo Transfection Kit
Altogen Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Our biology contract research services include: Xenograft services (over 60 in-house validated xenograft models), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
- 0.5 ml – 10 injections (Catalog #5060)
- 1.5 ml – 30 injections (Catalog #5061)
- 8.0 ml – 160 injections (Catalog #5062)
