Description
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ElectroporationKitforRamosCells (LymphomaCells,CRL-1596)
•ElectroporationKitinclude:electroporationbuffer+ buffer-specificoptimizedelectroporationprotocols
•Pre-optimizedelectroporationprotocolsareprovidedfordeliveryofplasmids(DNA), siRNA,mRNA,andproteins
•Proprietaryelectroporationbuffer formulationmimicscellularcytoplasmcomposition(superiorelectroporeresealing)s
•CertifiedRNAse-andDNAse-free electroporationbuffer
• Effective electroporationdeliveryandhighcellviABIlity providesreproducIBLeelectroporationresults
•Highcellviabilityandsuperiorcellrecoveryrates
• Electroporationprotocolsare providedwithwiththepurchaseoftheElectroporation Kit
•DevelopedandmanufacturedbyAltogenBiosystems
ElectroporationBuffer:
4mLofelectroporationbuffer issufficientfor20 reactions usingstandard0.2cmelecrtoporationcuvette
30mLofelectroporationbuffer issufficientfor150 reactions usingstandard0.2cmelecrtoporationcuvette
ElectroporationEfficiency:
Electroporationkitprovides 70-85%delivery efficiencyofsiRNA.Electroporation efficiencywasdeterminedbyqRT-PCR.
Electroporation ProtocolandMSDS:
ElectroporationprotocolsareprovidedwiththepurchaseoftheElectroporationKit
DownloadMSDS:[PDF]
RamosCellLine:
TheRamos(RA1)celllinewasestablishedfroma3-year-oldCaucasianmalepatientwhosufferedfromBurkitt’slymphoma.ThemorphologyofthiscelllineislikethatofalymphoblasttumOrigenictissue.ThesecellshavebeentestedasnegativeforEpstein-Barrvirus.Thesecellsareusefulforbiomedicalresearchrelatedtocancersofthelymphaticsystem.
Data:
RamosElectroporationKit
AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 4ml(Catalog#6583)
- 30ml(Catalog#6584)