Description
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Transfection Reagent for U87 MG Cells (Glioblastoma Cells)
- Two component formulation enhances lipid mediated transfection efficiency
- Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
- Kit includes Transfection Enhancer reagent and recommended transfection protocol
- High transfection efficacy in the presence of serum
- Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
- Download in vitro U87 transfection protocol: [PDF]
- Download U87 CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for U87 cells transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 81% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems U87 Transfection Protocol: [PDF]
Download MSDS: [PDF]
U-87 MG Cell Line:
Glioblastoma is a highly malignant tumor that originates from astrocytes and can be extremely difficult to eradicate due to it comprising many different cell types. Some of these cells react to treatment well, and others can be utterly unresponsive to drugs, as stated by the American Brain Tumor Association (ABTA). Human cell line models may lead to breakthroughs in brain cancer research and innovative treatment approaches that are essential for glioblastoma patients. U-87 MG is a human glioblastoma cell line derived from a stage three 44-year-old Caucasian female. It has epithelial morphology. Grade IV glioma, also called glioblastoma multiforme (GBM), is the most common primary malignant brain tumor with about 16,000 new diagnoses each year in the United States. GBMs have a one-year survival rate of only 29.6% making it one of the deadliest types of cancer. U-87 is a hypodiploid human cell line with the modal chromosome number of 44 that occurs in 48% of cells. The rate of higher ploidy is nearly 5.9%. Twelve markers are similar in all cells. There is only one copy of normal X, with N1, N6 and N9 being absent. U-87 MG cells are used as in vitro models of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The U-87 glioblastoma cell line is widely used in transfection studies and Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。 Altogen Biosystems全国代理部分产品列表： 货号 产品 规格 价格（美元） 5030 1703 A204 Transfection Reagent (Rhabdomyosarcoma) 0.5ml 170 1704 A204 Transfection Reagent (Rhabdomyosarcoma) 1.5ml 348 窗体顶端 窗体底端 2101 A204 Transfection Reagent (Rhabdomyosarcoma) 1.5ml CRISPR 548 窗体顶端 窗体底端 7018 A204 Transfection Reagent (Rhabdomyosarcoma) 8.0ml 1044 窗体顶端 窗体底端 1167 A375 Cells Transfection Reagent (CRL-1619) 0.5ml 195 1168 A375 Cells Transfection Reagent (CRL-1620) 1.5ml 427 2102 A375 Cells Transfection Reagent (CRL-1621) 1.5ml CRISPR 627 1169 A375 Cells Transfection Reagent (CRL-1622) 8.0ml 1281 5030 Nanoparticle In Vivo Transfection Reagent 0.5 ml - 10 injections 445 5010 LIPID In Vivo Transfection Reagent 0.5 ml - 10 injections 405 5040 PEG-Liposome In Vivo Transfection Reagent 0.5 ml - 10 injections 465 5020 POLYMER In Vivo Transfection Reagent 0.5 ml - 10 injections 425 5050 Pancreas In Vivo Transfection Kit 0.5 ml - 10 injections 495 5060 Liver In Vivo Transfection Kit 0.5 ml - 10 injections 495 5070 Kidney In Vivo Transfection Kit 0.5 ml - 10 injections 495 1721 BMS2 Transfection Reagent (Bone Marrow Cells) 0.5 ml 231 6345 C6 Transfection Reagent (Glioma Cells, CCL-107) 0.5 ml 199 6803 DLD-1 Transfection Reagent (Colon Cancer, CCL-221) 0.5 ml 201 1775 HUH-7 Transfection Reagent (Liver Cancer Cells) 0.5 ml 179
