Description
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TransfectionReagentforCT26.WTCells(ColonCarcinomaCells,CRL-2638)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- DownloadinvitroCT26WTtransfectionprotocol:[PDF]
- Download CT26.WTCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforCT26WTcellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast92%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsCT26WTTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
CT26.WTCellLine:
ColoncanceristhefourthmostcommoncancerdetectedintheUnitedStates.InadditiontobeingresponsIBLeforover95,000newcasesannually,ithasanincreasedincidencerateinpatientsyoungerthanage50,aspertheColonCancerAlliance(CCA).TheCT26.WTcoloncancercelllinewasderivedfromBALB/cmicethatdevelopedaggressivecoloncarcinomaasaresultofchemicallyinducedmutationandclonedwiththeretroviralvectorLXSN,containingthelacZgene.CT26.WTisahighlytumOrigeniccellline,whichiscommonlyemployedinthedrugdevelopmentforcoloncancer.Also,CT26.WTcellsareoftenusedasamodelforthetestingofimmunotherapyprotocols,aswellastransfectionhostcelllineforresearchstudiesontheimmuneresponse.AltogenBiosystemsprovideslipid-basedhigh-efficiencytransfectionreagentkitsfortheCT26.WTcelllinethathavedemonstratedconsistentresultsandlowcytotoxicity.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinCT26WTcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinCT26.WTcells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoCT26.WTcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
CT26.WTTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6454)
- 1.5ml(Catalog#6455)
- 1.5mlCRISPR(Catalog#2134)
- 8.0ml(Catalog#6456)