Description
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TransfectionReagentforBALB/3T3Cells (EmbryoFibroblastCells,CCL-163)
- Abiodegradablepolymerbasedtransfectionreagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- TransfectionkitincludesTransfectionEnhancerreagent
- Gentleenoughtobeusedforsinglecellanalysis
- DownloadinvitroBALB3T3transfectionprotocol:[PDF]
- Download BALB-3T3CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforBALB/3T3 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast79%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsBALB3T3TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
BALB/3T3CellLine:
Stemcelltherapyistheuseofstemcellsfortreatmentorpreventionofdifferentdiseases,includingstroke,spinalcordinjury,Alzheimer’sdisease,Parkinson’sdiseaseandotherneurologicalconditions.Connectivetissuecomprisesfibroblasts,activecellsthathelpmaintaintissuemetabolism.Mouseembryonicfibroblastsnourishembryonicstemcellsbysecretinggrowthfactorsinadditiontoprovidingacellularmatrixtopromotecellgrowth.Embryonicstemcellsareawidely-usedmeansforcellandmolecularBIOLOGyresearch,andmousefibroblastcelllinesareimperativeforthepropergrowthofstemcells.Whilebeingapowerfulresearchtool,thesecellsaresusceptIBLetoenvironmentalfactors;therefore,utmostcaremustbetakenwhileutilizingthemforresearch.Usingahigh-efficiencytransfectionreagentprovidesahigherdegreeofexperimentalreproducibilityandlowcytotoxicity.TheBALBcellline(alsoknownas3T3cloneA31)wasestablishedin1968fromfibroblasttissuesfoundinBALB/cmouse(Musmusculus)embryos.Thiscelllinecouldbeusefulforstudyingembryonictissueofmammals,suchasstemcellsandasatransfectionhostinavarietyofcellbiologyresearch.AltogenBiosystemssynthesizesbiodegradablepolymer-basedhigh-efficiencytransfectionreagentkitsfortheBALB/3T3mouseembryofibroblastcellline.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoBALB3T3cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminA/CinBALB/3T3cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoBALB/3T3cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
- Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
BALB/3T3TransfectionKit
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, elecroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenCROContractResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitwww.AltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6140)
- 1.5ml(Catalog#6141)
- 1.5mlCRISPR(Catalog#2112)
- 8.0ml(Catalog#6142)