Suspension Reagent to remove Hemoglobin interference for PCR
- Suitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed samples
- Scaleable suspension reagent
- Robust and simple, no phenol/chloroform or ammonium sulfate precipitation
- High Yield – free and soluble DNA or RNA is highly recoverable
- > 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cards
- Eliminates inhibitory effects of heme for PCR applications
- Compatible with silica-type final isolation preps
Some infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is designed to efficiently remove hemoglobin while maintaining very high recovery of nucleic acids, both RNA and DNA.
MATERIALS
Materials required | 5 ml | 50 ml |
HemogloBind™ Nucleic Acid suppliedas Suspension Reagent in 10 % Isopropanol | 5ml (25 prep) | 50ml (200 prep) |
Microfuge Spin-X type Filters | 25supplied | Recommended (Notsupplied) |
3M Guanidine ThioCyanate (orequivalent) | Notsupplied | Notsupplied |
Wide bore pipette tips | Suggested(Not Supplied) | |
HemogloBind™ Nucleic Acid 5ml (includes Spin filters) | H0250-05 |
HemogloBind™ Nucleic Acid 50ml (reagent only) | H0250-50 |
Click Here To View HemogloBind™ Product Sheet
Biotech Support Group Biotech Support Group LLC 成立于 2004 年。公司的使命是服务于蛋白质组样品制备的爆炸性增长，具有独特和专有的表面化学。致力于创造新的方法和应用，为所有下游蛋白质组学和生物标记物分析提供高效的工作流程和更好的数据质量。虽然选择性蛋白质结合是表面化学研究的热点，但其产物却可以交叉到其他「omic」领域，在基因组学、DNA 分离以及代谢组学等领域有着广泛的应用。 ELISA 试剂盒、试剂
