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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots
- Dried blood spots are useful for low volume analyses, and simple collection and transport
- Protocols suitable for inexpensive whole blood card systems, no need for cell separation
- Hemoglobin binding >90%, with 30-45 minute spin-filter format
- Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most volumes
- Blood proteins and enzymes are enriched for biomarker and proteomic investigations.
- Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat, mouse, etc.
- High throughput and scaleable.
Hemoglobin is a common contaminant from dried whole blood cards and not normally found in serum samples. The HemogloBind™ Blood Card protocol was designed to substantially reduce the presence of hemoglobin and its associated interference with many serum proteins and other analytes.
HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent sample prep in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin and cholinesterase analysis.
Hemoglobin depletion and Protein Recovery from Dried Blood Spots
| Dried Blood Spot (µl) | Hemoglobin Present (Based on 200mg Hb per ml blood) (mg) | Extraction Buffer (µl) | HemogloBind™ Reagent Used (ml) | Protein Recovery (µg) | Hemoglobin removal (%) |
|---|---|---|---|---|---|
| 20 | 4 | 200 | 200 | 400 - 500 | > 95% |
| 10 | 2 | 100 | 100 | 200 - 250 | > 95% |
| 5 | 1 | 50 | 50 | 100 - 125 | > 95% |
| 2.5 | 0.5 | 25 | 25 | 90 - 100 | > 95% |
| Product | # of samples processed | Item No. |
|---|---|---|
| HemogloBind™ Blood Card | 10 Dried Whole Blood Spot (7mm hole punch) | H0145BC-10 |
| HemogloBind™ Blood Card | 50 Dried Whole Blood Spot (7mm hole punch) | H0145BC-50 |
| Kit Content | 10 Prep | 50 Prep | Reagent |
|---|---|---|---|
| HemogloBind™ | 1 ml | 5 ml | Supplied |
| Protein Extraction Buffer (PEB) | 1 ml | 5 ml | Supplied |
| Spin-X Centrifuge tube filters | 10 | 50 | Supplied |
| Suggested Or Equivalent Supplier of Blood Card: Whatman 903™ Protein Saver cards | Not Supplied |
Click Here To View HemogloBind™ Blood Card Product Sheet
Biotech Support Group Biotech Support Group LLC 成立于 2004 年。公司的使命是服务于蛋白质组样品制备的爆炸性增长，具有独特和专有的表面化学。致力于创造新的方法和应用，为所有下游蛋白质组学和生物标记物分析提供高效的工作流程和更好的数据质量。虽然选择性蛋白质结合是表面化学研究的热点，但其产物却可以交叉到其他「omic」领域，在基因组学、DNA 分离以及代谢组学等领域有着广泛的应用。 ELISA 试剂盒、试剂
