Suspension Reagent to remove Hemoglobin interference for PCR
- Suitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed samples
- Scaleable suspension reagent
- Robust and simple, no phenol/chloroform or ammonium sulfate precipitation
- High Yield – free and soluble DNA or RNA is highly recoverable
- > 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cards
- Eliminates inhibitory effects of heme for PCR applications
- Compatible with silica-type final isolation preps
Some infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is designed to efficiently remove hemoglobin while maintaining very high recovery of nucleic acids, both RNA and DNA.
The protocol is easily scaled up or down to accommodate different starting blood volumes and works with common lysis buffers. Simply adjust the reagent volumes 5:1 proportionally to the starting blood volume.
Performance Efficiency | HemogloBind™ Nucleic AcidSuspension Reagent: SampleVolume Ratio | NucleicAcidRecovery |
RNA from Yeast, Total OD A260 = 15 | 5 : 1 | >95% |
Performance Efficiency | HemogloBind™ Nucleic AcidSuspension Reagent | Hemoglobin Removal | Protein Removal |
50 µl whole blood (Lysed using 3 M GuSCN. Final volume 150 µl.) | 250 µl | >95% | >95% |
Product | Item No. |
HemogloBind™ Nucleic Acid5ml (includes Spin filters) | HN250-20 |
HemogloBind™ Nucleic Acid50ml (reagent only) | HN250-200 |
MATERIALS
Materials required | HN250-20 | HN250-200 |
HemogloBind™ Nucleic Acid supplied as Suspension Reagent in <5 % Isopropanol | 5 ml | 50 ml |
No. Preps Based on 50 µl of lysed whole blood sample | 20 preps | 200 preps |
Microfuge Spin-X type Filters | 20supplied | Recommended(Not supplied) |
3M Guanidine ThioCyanate (or equivalent lysis) | Notsupplied | Notsupplied |
Wide bore pipette tips | Suggested(Not Supplied) | |
Click Here To View HemogloBind™ Nucleic Acid Product Sheet
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