Polymer Coated Silica Affinity Matrices For Glycoprotein Enrichment
- Non-specific sites are virtually eliminated by a polymer coating
- Stable across a wide pH range 4 – 10
- 1000Å, 50µm suitable for LC and batch processes
- Cis-diol specific, enriches heterogeneous sets of glycoproteins
- Unmasks glycoproteins from high abundance proteins, most notably albumin
- Efficient new surface technology ideal for proteomic applications
- Enriches glycoproteins from blood, serum, plasma, tissue or cell culture media.
- Sorbitol elution at pH 7.5 – 8.5
- Adaptable to liquid chromatography (LC) and high-throughput 96-well format
NuGel™ Phenyl Boronic Acid is a derivative of NuGel™ polyepoxy silica affinity support. This affinity support contains phenyl boronic groups at the end of hydrophilic spacer arms and is used to bind ligands containing cis-diols, glycoproteins.
NuGel™ Phenyl Boronic Acid Technical Data
Characteristics Of The Matrix | |
---|---|
Spacer Arm | Polymerized hydrophilic carbon chain |
Porosity | 1000Å |
Average Particle Size | 50um |
Special Features:
- Binds ligands containing 1,2 cis-diol groups of glycoproteins.
- pH stable from 4 to 10.