DNAzol®isacompleteandreadytousereagentfortheisolationofgenomicorviralDNAfromsolidandliquidsamplesofhuman,animalandplantorigin.TheDNAzolprocedureisbasedontheuseofanovelguanidine–detergentlysingsolutionthathydrolyzesRNAandpromotestheselectiveprecipitationofDNAfromthecelllysate(1,2).ThisadvancedDNAisolationmethodcombinesreliABIlityandefficiencywiththesimplicityofafastisolationprotocol.DNAzol®isanon-toxicsolutionandtheproceduredoesnotrequiretheuseofphenols.DNAcanbeobtainedfromalargenumberofsamplesofsmallorlargevolume;50mlofDNAzol®issufficienttoprocess50samples,eachwith25–50mgoftissue.GenomicDNAisolationcanbecompletedin10–30minuteswithDNArecoveryof70–100%.IsolatedDNAcanbeusedforSouthernanalysis,dotblothybridization,molecularcloningandpolymerasechainreaction.Thereare3versionsofDNAzol®,eachdesignedforoptimalisolationefficiencyfromvarioustypesofsamples.

DNAzol®(DN127)

$58.00–$452.00

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DNAzol®isforusewithtissues,cellsandliquidsamples.1.0mlofDNAzolisolatesDNAfrom25–50mgtissueorfromcellpelletscontaining1–3x10⁷cells.

SKU:DN127Category:DNAIsolation

DNAzol®ES(DN128)

$60.00–$476.00

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DNAzol®ESisextrastrengthDNAzolfortheisolationofgenomicDNAfromplants.1.5mlofDNAzol®ESisolatesDNAfrom0.5gofplanttissue.

SKU:DN128Category:DNAIsolation

DNAzol®BD(DN129)

$60.00–$476.00

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DNAzol®BDisspecificallydesignedfortheisolationofgenomicorviralDNAfromwholeblood.1.0mlofDNAzol®BDisolatesDNAfrom0.5mlofwholeblood.

SKU:DN129Category:DNAIsolation

References

USPatent5,945,515.

1. Chomczynski,P.,Mackey,K.,Drews,R.,andWilfinger,W.DNAzol®:AreagentfortherapidisolationofgenomicDNA.BioTechniques.22:550-553.1997.
2. Mackey,K.,Williams,P.,Seim,S.andChomczynski,P.TheuseofDNAzol®fortherapidisolationofgenomicDNAforwholeblood.Biomed.Products.Supplement:13-15.1996.