BcMag™ Cyanopropyl Magnetic Beads are uniform superparamagnetic resins containing cyanopropyl groups on their surface. The beads are specifically designed for quickly purifying, desalting, and concentrating femtomolar to the picomolar scale of very hydrophobic peptides or proteins that may be irreversibly retained on more hydrophobic C18 beads, manually or automatically without the need for a laborious repeat of pipetting and centrifugation.

Magnetic cyanopropyl (CN) bead is a mildly hydrophobic resin that can be used as a normal or reverse phase adsorbent. Under normal phase conditions, the polar component is removed from the non-polar organic solution. Under reversed-phase conditions, non-polar or weakly polar acid, medium, and basic biomolecules are removed from the aqueous solution. Furthermore, as a complex ligand, the cyanopropyl group can concentrate certain metal ions in an aqueous solution.

Hydrophobic interaction chromatography (HIC)

Hydrophobic interaction chromatography  (HIC) is a technique for separating macromolecules from one another based on a reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand (such as phenyl, octyl, or butyl) of a HIC medium. The interaction is enhanced by a buffer with a high salt concentration and reduced with a low salt concentration. Therefore, based on salt concentration in a buffer, the protein with less hydrophobicity is eluted first, whereas the protein with more hydrophobicity elutes last. Compared with other chromatography methods, HIC is a more popular method for separating and purifying protein and peptides in analytical and preparatory scale applications since it employs a less denaturing environment.

Hydrophobic magnetic beads

Bioclone hydrophobic magnetic resins are designed as uniform magnetic beads grafted with a high density of hydrophobic ligands on the surface. The hydrophobic magnetic beads are rigid polymeric beads with covalent surface chemistries, allowing easier handling and packing while providing more excellent physical and chemical stability—resulting in a robust production process. The beads replace time-consuming, difficult, and expensive chromatographic techniques such as agarose, cellulose, Sepharose, Sephadex-based columns, or resins. The hydrophobic magnetic beads are manufactured using nanometer-scale superparamagnetic iron oxide as core and entirely encapsulated by a high purity silica shell, ensuring no leaching problems with the iron oxide. The pure inert silica makes less nonspecific binding. The beads are much smaller (1 and 5 µm diameter) in size and are non-porous, which exhibit larger surface area, less nonspecific binding, and higher resolution than porous supports.

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MSDS

Related Hydrophobic Magnetic Beads → Bioclone的BcMag™NHS激活的磁珠是均匀的，基于二氧化硅的超顺磁珠，表面涂有高密度NHS（N-羟基琥珀酰亚胺）官能团。珠子用于特异性地缀合含伯胺的配体（图1），因此在偶联之前不需要保护配体中的其他活性基团。耦合快（室温pH 6.5–9 15–30分钟，4°C 4小时）。BcMag™NHS激活的磁珠最适合与大蛋白结合。建议使用BcMagTM长臂（17 atome）NHS激活的磁珠来缀合小肽，因为长臂（21原子）亲水性接头可以减少空间位阻。