Hydrophobic interaction chromatography (HIC) is a technique employed for the segregation of macromolecules by exploiting the reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand of a HIC medium, such as phenyl, octyl, or butyl. The interaction is amplified in the presence of a buffer with a high salt concentration, and conversely reduced with a low salt concentration. As a result, based on the salt concentration in the buffer, the protein with less hydrophobicity tends to elute first, whereas the protein with more hydrophobicity elutes last. This method is gaining popularity over other chromatography methods in both analytical and preparatory scale applications, owing to its usage of a less denaturing environment.

The BcMag™ Phenyl Magnetic Beads are distinguished by their homogeneity and superparamagnetic properties. They possess hydrophobic entities on their exterior that expedite their effectiveness as a chromatographic matrix. These beads are meticulously crafted to allow for manual or automatic purification, desalination, and concentration of proteins or peptides within the femtomolar to picomolar range, consequently eliminating the need for repetitive pipetting and centrifugation procedures that can be laborious and time-consuming. Additionally, the BcMag™ Phenyl Magnetic Beads, like C8 beads, share similar polarity; however, they incorporate an electron-rich aromatic ring, which provides exclusive selectivity and retention.

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MSDS

Related Hydrophobic Magnetic Beads → Bioclone的BcMag™NHS激活的磁珠是均匀的，基于二氧化硅的超顺磁珠，表面涂有高密度NHS（N-羟基琥珀酰亚胺）官能团。珠子用于特异性地缀合含伯胺的配体（图1），因此在偶联之前不需要保护配体中的其他活性基团。耦合快（室温pH 6.5–9 15–30分钟，4°C 4小时）。BcMag™NHS激活的磁珠最适合与大蛋白结合。建议使用BcMagTM长臂（17 atome）NHS激活的磁珠来缀合小肽，因为长臂（21原子）亲水性接头可以减少空间位阻。