In the field of biological research, the isolation and purification of specific molecules such as proteins and nucleic acids are crucial to understanding their function and structure. Traditional methods of chromatography, such as ion exchange chromatography, have been the standard in separating these molecules from crude biological samples. However, the process can be time-consuming and require extensive expertise. Magnetic beads, coupled with adsorbent technology, provide a faster and more efficient alternative for fractionating biological molecules.
BcMag™ PEI Magnetic Beads function as powerful Anion Exchange resins. The magnetic beads possess a robust PEI strong anion exchange format, enabling prompt and substantial processing of up to 96 samples in a mere 20-minute span. The technology allows for rapid and efficient fractionation of proteins or nucleic acids from complex biological specimens, such as serum and plasma, either manually or automatically. The refined protein may then be employed in downstream applications, including sample fractionation for 1D and 2D SDS-PAGE, X-ray crystallization, and NMR spectroscopy. Furthermore, strong ion exchangers can prove to be valuable separation tools when weak ion exchangers fall short, owing to the distinct selectivity of weak and strong ion exchangers.
Strong anion exchange beads feature and benefits
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Fast and simple – PEI magnetic beads-based format eliminates columns or filters or a laborious repeat of pipetting or centrifugation.
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Convenient and expandable – PEI magnetic format enables high-throughput processing of multiple samples in parallel with many different automated liquid handling systems.
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Robust – PEI magnetic beads do not crack or run dry.
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Low bed volume – Working with small magnetic bead volumes allows for minimal buffer volumes, resulting in concentrated elution fractions.
Strong anion exchange beads Applications
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Protein pre-fractionation in cell lysates
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Optimizing purification conditions for new protein preparation protocols
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Protein purification and concentration
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Antibody purification from serum, ascites, or tissue culture supernatant
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Preparation of samples before 1D or 2D PAGE
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Phosphopeptide purification before MS analysis
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Instruction Manual
MSDS
Related Ion Exchange Magnetic Beads → Bioclone的BcMag™NHS激活的磁珠是均匀的，基于二氧化硅的超顺磁珠，表面涂有高密度NHS（N-羟基琥珀酰亚胺）官能团。珠子用于特异性地缀合含伯胺的配体（图1），因此在偶联之前不需要保护配体中的其他活性基团。耦合快（室温pH 6.5–9 15–30分钟，4°C 4小时）。BcMag™NHS激活的磁珠最适合与大蛋白结合。建议使用BcMagTM长臂（17 atome）NHS激活的磁珠来缀合小肽，因为长臂（21原子）亲水性接头可以减少空间位阻。
