Magnetic Beads fractionate proteins or nucleic acids using beads-adsorbent technology as a chromatographic matrix. Ion exchange chromatography is widely used to separate or purify a target molecule from crude biological materials. The molecules are separated based on variations in their accessible surface charges utilizing very light binding and eluting conditions for intact biological activity.
The PSA (N-propylethylenediamine) bead, like the NH2 magnetic beads, has two amino groups (primary and secondary amines). The two amino groups have higher pKa values (10.1 and 10.9, respectively), so the PSA beads have a higher ion exchange capacity and stronger ion exchange capacity. At the same time, the PSA bonded phase as bidentate ligands, which can produce chelation, a chemical reaction useful in, as a result, PSA has less polar.
BcMag™ PSA Magnetic Beads are uniform magnetic resins grafted with a high density of N- PSA (N-propylethylenediamine) groups on the surface (Fig.1). The weak anion exchange magnetic bead-based format enables rapid high-yield processing of 96 samples in about 20 minutes. It can quickly fraction proteins or nucleic acids from complex biological samples (such as serum, plasma, etc.) manually or automatically. The purified protein can be used in downstream applications such as sample fractionation for 1D and 2D SDS-PAGE, X-ray crystallization, and NMR spectroscopy. The Weak Ion Exchange resins allow the rapid release of very strong ions that may be retained irreversibly on Strong Ion Exchange beads. Additionally, weak ion exchangers can be effective separation tools when strong ion exchangers fail because the selectivity of weak and strong ion exchangers frequently differ.
Weak anion exchange magnetic resins are used to replace time-consuming, complex, and costly chromatographic procedures such as agarose, cellulose, Sepharose, and Sephadex-based columns or resins. In column-based procedures, the lysate is centrifuged or cleared, the supernatant is added to the column, the membrane or resin is washed with buffer through centrifugation or vacuum manifold, and the required biomolecules are eluted in an adequate volume of buffer. When using column-based technologies, processing multiple samples in academic research labs may necessitate a significant quantity of hand pipetting. This pipetting can discourage differences in target biomolecule yield between experiments and people. Staff and students may require extensive training and practice to produce constant protein yields.
Weak anion exchange magnetic resins have significant advantages over non-magnetic resin technologies. It is due to the numerous benefits of magnetic resins, such as their ease of use, rapid experimental protocols, suitability, and convenience for high throughput automated and miniaturized processing. They thus see increasing use in various areas of life-sciences research and development, including drug discovery, biomedicine, bioassay development, diagnostics, genomics, and proteomics.
Weak anion exchange beads feature and benefits
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Fast and straightforward – PSA magnetic beads-based format eliminates columns or filters or a laborious repeat of pipetting or centrifugation.
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Convenient and expandable – Magnetic format enables high-throughput processing of multiple samples in parallel with many different automated liquid handling systems.
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Robust – PSA Magnetic beads do not crack or run dry.
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Low bed volume – Working with small magnetic bead volumes allows for minimal buffer volumes, resulting in concentrated elution fractions.
PSA magnetic beads Applications
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Protein pre-fractionation in cell lysates
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Optimizing purification conditions for new protein preparation protocols
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Protein purification and concentration
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Antibody purification from serum, ascites, or tissue culture supernatant
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Preparation of samples before 1D or 2D PAGE
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Phosphopeptide purification before MS analysis
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Instruction Manual
MSDS
Related Ion Exchange Magnetic Beads →General Reference
1.
Dasgupta PK, Maleki F. Ion exchange membranes in ion chromatography and related applications. Talanta. 2019 Nov 1;204:89-137
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Wittkopp F, Peek L, Hafner M, Frech C. Modeling and simulation of protein elution in linear pH and salt gradients on weak, strong and mixed cation exchange resins applying an extended Donnan ion exchange model. J Chromatogr A. 2018 Apr 13;1545:32-47.
3.
Staby A, Jensen RH, Bensch M, Hubbuch J, Dünweber DL, Krarup J, Nielsen J, Lund M, Kidal S, Hansen TB, Jensen IH. Comparison of chromatographic ion-exchange resins VI. Weak anion-exchange resins. J Chromatogr A. 2007 Sep 14;1164(1-2):82-94.
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Staby A, Jacobsen JH, Hansen RG, Bruus UK, Jensen IH. Comparison of chromatographic ion-exchange resins V. Strong and weak cation-exchange resins. J Chromatogr A. 2006 Jun 23;1118(2):168-79.
5.
Fishman JB, Berg EA. Purification of Antibodies: diethylaminoethyl (DEAE) Chromatography. Cold Spring Harb Protoc. 2019 Jan 2;2019(1).
6.
Černigoj U, Vidič J, Ferjančič A, Sinur U, Božič K, Mencin N, Martinčič Celjar A, Gagnon P, Štrancar A. Guanidine improves DEAE anion exchange-based analytical separation of plasmid DNA. Electrophoresis. 2021 Dec;42(24):2619-2625.
7.
Shields PA, Farrah SR. Characterization of virus adsorption by using DEAE-sepharose and octyl-sepharose. Appl Environ Microbiol. 2002 Aug;68(8):3965-8.
Bioclone的BcMag™NHS激活的磁珠是均匀的，基于二氧化硅的超顺磁珠，表面涂有高密度NHS（N-羟基琥珀酰亚胺）官能团。珠子用于特异性地缀合含伯胺的配体（图1），因此在偶联之前不需要保护配体中的其他活性基团。耦合快（室温pH 6.5–9 15–30分钟，4°C 4小时）。BcMag™NHS激活的磁珠最适合与大蛋白结合。建议使用BcMagTM长臂（17 atome）NHS激活的磁珠来缀合小肽，因为长臂（21原子）亲水性接头可以减少空间位阻。
