A sulfhydryl is a functional group that contains a sulfur atom linked to a hydrogen atom. In chemistry, the sulfhydryl group, often known as a thiol, is denoted by the suffix “-thiol” and the prefix “mercapto-” or “sulfanyl.” Thiols have a strong preference for soft metals.

BcMag™ Sulfhydryl-Terminated Magnetic Beads are uniform, silica-based superparamagnetic beads grafted with a high density of sulfhydryl (SH) functional groups on the surface. The Sulfhydryl-Magnetic Beads are specially designed for conjugation of protein/peptides modified by SMCC (Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate). SMCC contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The procedure is quick and straightforward for sample preparation without laborious repeat pipetting and centrifuging. BcMagTM Sulfhydryl-Terminated Magnetic Beads are ideal for conjugating large proteins. BcMagTM Long-Arm Sulfhydryl-Terminated Magnetic Beads are recommended for conjugating small peptides.

Workflow

BcMag™ Sulfhydryl-Terminated Magnetic Beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.

Learn More

Instruction Manual

MSDS

Related Affinity Magnetic Beads  → Bioclone的BcMag™NHS激活的磁珠是均匀的，基于二氧化硅的超顺磁珠，表面涂有高密度NHS（N-羟基琥珀酰亚胺）官能团。珠子用于特异性地缀合含伯胺的配体（图1），因此在偶联之前不需要保护配体中的其他活性基团。耦合快（室温pH 6.5–9 15–30分钟，4°C 4小时）。BcMag™NHS激活的磁珠最适合与大蛋白结合。建议使用BcMagTM长臂（17 atome）NHS激活的磁珠来缀合小肽，因为长臂（21原子）亲水性接头可以减少空间位阻。