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商品描述
ApolipoproteinB | |
| Manufacturer/Trade: | Exocell/ApoBTest |
| CatalogueNumbers: | 1006StripPlate |
| Methodology: | IndirectCompetitiveELISA |
| Summaryofprocedure: | TheApoBTestisdesignedforthequantitativemeasurementofapolipoproteinBinhumanplasma.Tocompletetheassay,B36monoclonalantibodyisaddedtoamicrotiterplatewellthatcontainsapolipoproteinBimmobilizedtothesolidphase,andtowhichapolipoproteinB,standardorsample,isaddedinthefluidphase.Duringa30minuteincubation,themonoclonalantibodybindstoeithertheimmobilizedapoBortothesolubleapoB,hencethenotionofcompetitivebinding.Unboundreactantsarethenwashedaway,andasecondincubationof15minuteswithanenzymeconjugatedtoananti-mouseantibodyreactswiththeB36thatwascapturedbythesolidphase.Thewellsarewashedtoremoveunboundsecondaryantibody,andTMBSubstratesolutionisaddedtodetectboundconjugate.Thechromogenicreactionisstoppedbyanadditionofdiluteacid,andcolorintensitydeterminedbyabsorbanceat450nm.Sampleconcentrationisdeterminedbyrelatingresponsetothatforknownstandards. |
| SpecimenRequired: | 10ulcitrateorEDTAtreatedplasma |
| AssayRange: | 0.13-2.0mg/ml(correspondingto13-200mg/dl) |
| Precision: | Intra-andinterassayprecisionofsampleswithintheassayrangehaveaC.V.<10%. |
| Specificity: | ThemonoclonalantibodyB36isspecificforhumanapolipoproteinB,anddoesnotcross-reactwithotherproteinsorsubstancesfoundinhumanplasma. |
| ExpectedValues: | ExpectedvaluesforapoBinmalesubjectsarereportedtobe108+/-33mg/dlforindividualsfreeofcoronaryarterydisease(CAD),and131+/-37forsubjectswithangiographicallydocumentedprematureCAD.Valuesforwomenaresimilartothoseformen. |
 ©2012ExocellInc. | |
