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商品描述
mAlbumin | |
| Manufacturer/Trade: | Exocell/AlbuwellM |
| CatalogueNumbers: | 1011StripPlate |
| Methodology: | CompetitiveELISA |
| Summaryofprocedure: | AlbuwellMisadirectcompetitiveELISA(antibodycapture)designedtomonitorkidneyfunctioninthemousebymeasuringurinaryalbumin.Tocompletetheassay,sampleandanti-mousealbuminAb-HRPconjugateareaddedtoalbumincoatedwells.Theantibodyconjugatereactswitheitherthealbumincoatedtotheplateorwiththatinthefluidphase,hencethenotionofcompetitivebinding. Aftera30minuteincubation,theplateiswashedtoremovereactantsinthefluidphase.Onlyconjugatethatwasboundtothealbuminontheplateremains,anditisdetectedusingTMBinachromogenicreaction.Colorintensityisinverselyproportionaltothelogarithmofthealbuminconcentrationinthefluidphase.Theassaymaybecompletedinlessthananhour. |
| SpecimenRequired: | Urine,10ul |
| AssayRange: | 0.156-10ug/mL |
| Precision: | IntrassayandinterassayprecisionforsampleswithinusefulrangeoftheassayhaveaC.V.≤10%ofthemean. |
 ©2016ExocellInc. | |
