4% B Agarose Bead Standard is a non-crosslinked agarose resin with a bead size of 50-150 µm. It is a resin commonly used for gel filtration as well coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.
REFERENCES:
- Franziska Hagemann, Patrick Adametz, Matthias Wessling, Volkmar Thom, "Modeling hindered diffusion of antibodies in agarose beads considering pore size reduction due to
adsorption." Journal of Chromatography A (2020) - Kato, Shinji et al. “The Membrane Phospholipid Cardiolipin Plays a Pivotal Role in Bile Acid Adaptation by Lactobacillus Gasseri JCM1131 T.” Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1864.3 (2019): 403–412. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. Web.
Product Name | 4% B Agarose Bead Standard (50-150µm) |
Cat. No. | A-1040S-X |
Bead Geometry & Size | Spherical ~50-150 µm |
Bead Mean Diameter d50v | ~90 µm |
Crosslinked | No |
Agarose % | 4% |
Linear Recommended Flow Rate | <11.5>11.5> |
Fractionation (mw) Globular Proteins Da | 7x10^4 - 2x10^7 |
Exclusion Limit Da | > 2x10^7 |
pH Stability Working Range | 4-9 |
ph stability cleaning in place (cip) | 4-9 |
Chemical Stability | Stable in moderate acid and basic solutions |
Antimicrobial Agent | 20% Ethanol |
Storage Temperature | 2-30˚C |