Description

Alumen450™isaturn-onfluorescentsensor,meaningthatthesolutionfluorescenceincreasesasafunctionoftheconcentrationofdissolvedAluminum.Alumen450™canreADIlybeexcitedoverabroadwavelengthrangefrom280-400nmwithanextinctioncoefficientof8300±600M^-1cm^-1at318nm.ThefluorescenceemissionisverystronginthepresenceofAluminumions,centeredat ≈420nm.

Alumen450™readychelatesdissolvedAl³⁺andsubsequentlyshowsanincreaseintheemissionspectraforAlumenBlue™inthepresenceofdissolvedAl³⁺,i.e.itgetssignificantlybrighter. Theprobeincreasesitsfluorescenceemission>300%ingoingfrom0 →750µMAl³⁺. Alumen450™showsanalmostlinearresponsetoAl³⁺from1 →300µMdissolvedAl³⁺.Alumen450™isalsoahighlyselectiveprobeforboththedetectionanddeterminationofdissolvedAl³⁺overotherdissolvedcations.

Alumen450™issolddryandissimplyreconstitutedbyaddingwater/MeOH/EtOHand/orbuffer.Werecommenda ≈95/5%ethanol/watermixture(v/v).Onevialofourprobewilltypicallyallowfor10aluminumsensingmeasurementstobeundertaken;50µLofsolventistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm³(a3mlvolume)separately,typicallygiveanopticaldensity>0.1at360nm.

Foroptimumperformance,probevialsshouldbestoredat-2to-8°Cwhennotbeingusedand< - 20 °C long term. Probe solutions should be used after dilution.

AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.

Ursa BioScience提供了一系列具有不同水溶性的高量子产率荧光团，可用于各种客户的氯离子传感应用。我们的氯化物探针可通过存在水溶液，络合物或假氯化物而容易地进行荧光淬灭（强度和寿命），非常适合于生理或环境中氯化物的测定。