Description
MellitusBlueC™istheconvenientcontrolcompoundforMellitusBlueprobes1-3™.Withthesamechromophorefluorescentbackbone,butdoesnotchelatedissolvedglucose,MellitusBlueC™servesasanidealcontrolcompoundforall3oftheMellitusBlue™probes.MellitusBlueC™doesnotshowanyresponsetoglucose,fructoseorotherdiols.Similartotheparentprobemolecules,MellitusBlueC™canreADIlybeexcitedfrom300-400nm,withanemissioncentredat≈460nmintheblueregionofthevisIBLespectrum.
MellitusBlueC™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourprobewilltypicallyallowfor10control-likemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette.50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
Ursa BioScience提供了一系列具有不同水溶性的高量子产率荧光团,可用于各种客户的氯离子传感应用。我们的氯化物探针可通过存在水溶液,络合物或假氯化物而容易地进行荧光淬灭(强度和寿命),非常适合于生理或环境中氯化物的测定。