Description
MellitusBlue2™showsafluorescenceintensityreductionasafunctionofglucoseconcentrationupto100mMglucose,withaglucosepKaof6.90and5.00for100mMFructose.MellitusBlue2™canreADIlybeexcitedfrom300-400nmwithanemission≈460nm.
MellitusBlue2™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourglucoseprobewilltypicallyallowfor10glucosemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette.50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.WerecommendtheuseofMellitusBlueC™,thecontrolcompoundforMellitusBlue2™,totestforpossIBLebackgroundinterferences,suchasfromfructose,othersugarsanddiolinterference.
Ursa BioScience提供了一系列具有不同水溶性的高量子产率荧光团，可用于各种客户的氯离子传感应用。我们的氯化物探针可通过存在水溶液，络合物或假氯化物而容易地进行荧光淬灭（强度和寿命），非常适合于生理或环境中氯化物的测定。
