Description
PhosYellow™isahighlyphosphorescentorganicprobewhichcanbeexcitedfrom300-380nm.Inairequilibratedglycerol,thefluorescenceemissionoccursat≈400nmandthephosphorescenceiscentered≈540nm,withafwhmof≈100nm.Thephosphorescencelifetimeinairequilibratedglycerolat22C(em=525nm)is≈120µS.PhosYellow™isreADIlysolubleinmethanol.ethanolandacetone.
PhosYellow™issolddryandissimplyreconstitutedbyaddingMeOHorEtOHorothersuitablesolvent.Onevialofthisprobewilltypicallyallowfor10measurementstobeundertaken: 50µLofMeOHistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3(cuvette),willtypicallygiveanopticaldensity>0.05at320nm(inspectroscopicMeOH).
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.
Ursa BioScience提供了一系列具有不同水溶性的高量子产率荧光团,可用于各种客户的氯离子传感应用。我们的氯化物探针可通过存在水溶液,络合物或假氯化物而容易地进行荧光淬灭(强度和寿命),非常适合于生理或环境中氯化物的测定。