Description
DualVpH™isawatersolubledualpHandviscosityprobe,excitation300-400nm,andaviscositydependentemissioncenteredaround400nm.Thequantumyieldislowinaqueousmedia(≈1cP),butincreasesastheviscosityincreasesmakingitanidealviscositycontrastprobe.
DualVpH™issolddryandissimplyreconstitutedbyaddingwater/MeOH/EtOHand/orbuffer.OnevialofourViscosityProbewilltypicallyallowfor10measurementstobeundertaken: 50µLofwaterorMeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3,typicallygiveanopticaldensity>0.1at340nm(inspectroscopicMeOH).
Foroptimumperformance,probevialsshouldbestoredat-2to-8°Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
Ursa BioScience提供了一系列具有不同水溶性的高量子产率荧光团,可用于各种客户的氯离子传感应用。我们的氯化物探针可通过存在水溶液,络合物或假氯化物而容易地进行荧光淬灭(强度和寿命),非常适合于生理或环境中氯化物的测定。