Overview

Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:

Lentivector-based for transfection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.

PiggyBac Transposon-based* for infection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.Pre-built Jurkat cell line to save time and labor

Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.

*Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.