Clearly visualize cells in vivo and ex vivo for tracking cell fate and more

Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.

SBI"s lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models.

The CMV-Luciferase-RFP-TK Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. Because the vector becomes integrated into the genome, you can use this vector to create stable reporter cell lines.

The vector expresses RFP, luciferase, and TK from a CMV promoter for strong expression in most common cell types, such as HeLa, HEK293, or HT1080. Available as either a plasmid or pre-packaged virus.

Which BLIV construct should you choose?

With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:

Choose a lentivector when:

• You’re working with difficult-to-transfect cells
• You’d like to create stable reporter cell lines
• You’d like to integrate your reporter into the genome

Choose a minicircle vector* when you’re working with transfectable cells and:

• You’d like episomal expression
• You’d like to avoid introducing foreign sequences

Choose your promoter using the following table:

Promoter	Expression Level	Application
CMV	High	Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV	High	Hematopoietic and stem cells
EF1α	Medium	Most cell types including primary cells and stem cells
PGK	Medium	Most cell types including primary cells and stem cells
UbC	Low	Most cell types including primary cells and stem cells

*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.

SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应