
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
View Products
A quick new way to study protein function in vivo
With SBI’s pCDH-CMV-Piranha™-EF1-GFP-T2A-Puro Pre-pacakged Lentivirus, you can build your own cell line constitutively expressing the Piranha Targeted Protein Degradation System. In this ready-to-transduce lentivirus, the Piranha protein is expressed from the strong CMV promoter and the construct is marked with GFP and puromycin. To initiate targeted protein degradation in your Piranha-expressing cell line, simply electroporate in antibody specific for your protein-of-interest—you’ll start seeing degradation of the protein-of-interest in as little as 1 hour*.
While studying protein function in vivo often involves knocking down expression using siRNA or knocking out the gene via CRISPR/Cas9, these approaches are indirect and often imperfect. siRNA can reduce or eliminate protein expression, but if the protein turn-over rate is slow then it can take days to see a reduction in function. Simply knocking out the gene coding for the protein-of-interest is not always possible, limits the type of studies you can do, and is incompatible with essential genes. To give researchers another faster option, SBI developed the Piranha Targeted Protein Degradation System.
*Based on published data by Clift et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017 Dec 14;171(7):1692-1706.e18. doi: 10.1016/j.cell.2017.10.033. Degradation rate of target protein and resulting phenotypic effects will depend on the specific protein being targeted and influenced by protein degradation pathway and any compensatory mechanisms. An initial time course experiment to find optimal conditions to assay for protein knockdown and phenotypes is highly recommended.
Available Piranha Targeted Protein Degradation System Products
Table 1. Available Piranha Targeted Protein Degradation System Products
Cat. # | Product |
---|---|
Electroporation-ready mRNA | |
PTPD500A-1 | Piranha Electroporation-ready untagged mRNA |
PTPD510A-1 | Piranha Electroporation-ready RFP-tagged mRNA |
PTPD520A-1 | Piranha Electroporation-ready GFP-tagged mRNA |
Stable Cell Line | |
PTPD600A-1 | Piranha HEK293T GFP/Puromycin Stable Cell Line |
Pre-packaged Lentivirus | |
PTPD513VA-1 | pCDH-CMV-Piranha-EF1-GFP-T2A-Puro Pre-packaged Lentivirus |
PTPD527VA-1 | pCDH-EF1a-Piranha-T2A-Puro Pre-packaged Lentivirus |
资质认证
获得国家资质,权威认证!
全国联保
全国联保,官方无忧售后
正规发票
正规发票,放心购买
签订合同
签订合同,保障您的权益