Faster insights into protein function in vivo

Quickly and directly study the role a protein plays in vivo with SBI’s Piranha Targeted Protein Degradation System, Electroporation-ready mRNA. After electroporating both the Piranha mRNA and an antibody specific for your protein-of-interest into target cells, you can start seeing degradation of the protein-of-interest in as little as 1 hour*. Piranha Electroporation-ready mRNA is available in untagged, RFP-tagged, or GFP-tagged versions (also available as Pre-packaged Lentivirus or HEK293T Stable Cell Line).

While studying protein function in vivo often involves knocking down expression using siRNA or knocking out the gene via CRISPR/Cas9, these approaches are indirect and often imperfect. siRNA can reduce or eliminate protein expression, but if the protein turn-over rate is slow then it can take days to see a reduction in function. Simply knocking out the gene coding for the protein-of-interest is not always possible, limits the type of studies you can do, and is incompatible with essential genes. To give researchers another faster option, SBI developed the Piranha Targeted Protein Degradation System.

• Specific—avoid off-target effects associated with genomic-based approaches (CRISPR/Cas9) or mRNA targeting (RNAi) by directly targeting the protein-of-interest
• Fast—observe protein degradation in as little as 1 hour* for speedier, more efficient studies
• Simple—relies on only two reagents, the Piranha System and your validated antibody
• Powerful—antibody-based targeting means the Piranha System can selectively degrade the phosphorylated or other post-translationally modified form of a protein while leaving the unmodified form intact
• Scalable for screening—use either the HEK293T Piranha Cell Line or your own cell line stably expressing Piranha protein to evaluate multiple protein targets in parallel (96- or 384-well plate assays)
• Unique—the only commercially available system for direct targeted degradation of protein(s)-of-interest for phenotypic studies
• Flexible—available as electroporation-ready mRNA, constitutively expressed in a HEK293T cell line, or as pre-packaged lentivirus for stable Piranha expression in the cell line of your choice

*Based on published data by Clift et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017 Dec 14;171(7):1692-1706.e18. doi: 10.1016/j.cell.2017.10.033.  Degradation rate of target protein and resulting phenotypic effects will depend on the specific protein being targeted and influenced by protein degradation pathway and any compensatory mechanisms.  An initial time course experiment to find optimal conditions to assay for protein knockdown and phenotypes is highly recommended.

Available Piranha Targeted Protein Degradation System Products

Table 1. Available Piranha Targeted Protein Degradation System Products

Cat. #	Product
Electroporation-ready mRNA
PTPD500A-1	Piranha Electroporation-ready untagged mRNA
PTPD510A-1	Piranha Electroporation-ready RFP-tagged mRNA
PTPD520A-1	Piranha Electroporation-ready GFP-tagged mRNA
Stable Cell Line
PTPD600A-1	Piranha HEK293T GFP/Puromycin Stable Cell Line
Pre-packaged Lentivirus
PTPD513VA-1	pCDH-CMV-Piranha-EF1-GFP-T2A-Puro Pre-packaged Lentivirus
PTPD527VA-1	pCDH-EF1a-Piranha-T2A-Puro Pre-packaged Lentivirus

SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应