Gain the confidence to move forward with informative controlsWhile lentiviral transduction can be a powerful and effective method for delivering expression cassettes to target cells, enabling stable expression of genes, miRNAs, shRNAs, etc., some cell lines are transduced less efficiently than others. To help with lentiviral transduction optimization and troubleshooting, SBI offers a series of positive transduction controls that come as pre-packaged, ready-to-transduce lentiviral particles as well as ready-to-transfect and package lentivector plasmids. The pSIH1-H1-siLuc-copGFP Positive Transduction Control enables quantitation of HIV-based lentiviral transduction efficiency using the bright copGFP protein.

• Easily visualize and quantify transduction efficiency with copGFP
• Optimize transduction conditions for your cell line
• Test the sensitivity of target cells to HIV-based lentiviral transduction

pSIH1-H1-siLuc-copGFP expresses the full-length copGFP gene with human-optimized codons from the CMV promoter for high levels of expression in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepods (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation and high stability at a wide range of pH (pH 4 – 12). copGFP does not require any additional cofactors or substrates and has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:

• Maximum emission wavelength: 502 nm
• Maximum excitation wavelength: 482 nm
• Quantum yield: 0.6
• Extinction coefficient: 70,000 M^-1 cm^-1

SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应