A kit that streamlines genome editing at the powerful AAVS1 Safe Harbor Site

Get the critical reagents for knocking-in any gene or promoter-gene combination into the AAVS1 Safe Harbor Site with this Kit that bundles together an HR Donor—the AAVS1-Targeting All-purpose HR Donor Vector 2.0 AAVS1-SA-puro-MCS—a Cas9/AAVS1 gRNA delivery construct—the All-in-one Cas9 SmartNuclease & AAVS1 gRNA Plasmid—and a 5’ and 3’ primer mix for a Junction PCR Assay to verify integration into the AAVS1 site.

Our second generation, All-purpose HR Donor Vector 2.0 AAVS1-SA-puro-MCS is a promoterless construct enabling knock-in of any gene or promoter-gene combination into the AAVS1 site. Like all of our AAVS1 HR Donor Vectors, it come with AAVS1 homology arms already cloned in, simplifying your workflow. Just ligate-in the gene or promoter-gene combination of your choice and co-transfect with the included All-in-one Cas9 SmartNuclease & AAVS1 gRNA Plasmid. You can verify correct insertion with the included 5’ and 3’ Primer Mix for Junction PCR Assay.

Why choose one of our second generation AAVS1-targeting HR Donors?

The clever design of our second generation AAVS1-Targeting HR Donor Vectors limits off-target integration for highly-specific targeting of the AAVS1 site. Taking advantage of the AAVS1"s location within an intron, the puromycin marker has only a splice acceptor site and no promoter. Expression of puromycin can only occur when the construct integrates within an intron, reducing the probability of recovering off-target integrants in the presence of puromycin selection.

Why AAVS1?

Delivering consistent, robust transgene expression, the AAVS1 safe harbor site is a preferred target for gene knock-ins. Insertion at the site has been shown to be safe with no phenotypic effects reported, and the surrounding DNA appears to be kept in an open confirmation, enabling stable expression of a variety of transgenes.

SBI’s AAVS1 Safe Harbor Targeting products deliver:

• Easy, precise knock-in of any gene
• Consistent, robust transgene expression from the AAVS1 Safe Harbor Site
• Simplified construction of isogenic cell lines
• Minimal off-target integration
• Streamlined CRISPR/Cas9 library screening

Why use an HR targeting vector?

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.

SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应