Enhance your All-in-one Cas9 SmartNickase Plasmid with multiplexed gRNA delivery

Easily enhance the power of the CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid by combining it with the Multiplex gRNA Cloning Kit. Together, these two products can enable a wider range of genome editing projects that require multiple gRNAs.

You get all the great features of the CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid:

• Conveniently deliver Cas9 and gRNA with a single vector
• Reduce off-target activity
• Drive Cas9 expression with the CAG promoter, which provides high expression levels in primary cells and stem cells
• Express gRNA from the H1 promoter for maximum specificity and choice of targets
• Ensure efficient import of Cas9 to the nucleus with N-term and C-term nuclear localization signals (NLSs)
• Boost Cas9 gene expression and stabilize the transcript via the WPRE regulatory element after the C-term NLS
• Easily detect and/or purify the Cas9 protein with the N-term myc-tag
• Produce Cas9 mRNA via in vitro transcription using the T7 promoter

Combined with the advantages of the Multiplex gRNA Cloning Kit:

• Edit multiple loci at once, save time and reagents
• Easily generate multi-cistronic gRNA expression constructs
• Ideal for Cas9 Nickase applications
• Great for studying signaling pathways

As with all of our Cas9 delivery options, the CAG-T7-hspCas9-Nickase-H1-gRNA Plasmid is functionally validated and comes backed by our expert technical support team—if you’ve got a genome engineering question just ask by emailing tech@systembio.com.

Why an HR targeting vector is a recommended

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.

Not sure whether you need a CRISPR/Cas9 plasmid, purified protein, or mRNA?

Use this table to choose the CRISPR/Cas9 product that’s right for you:

For This Application	In these types of cells

Use These ProductsMODIFYING ORGANISMS

• Gene tagging


• Transgenic organism generation


• Model organism engineering

Embryos—to create transgenic animalsInjectable Cas9 mRNA & gRNA Synthesis Kits
Cas9 Protein
EGFP-labeled Cas9 ProteinAnimals models—in vivo genome editingAAV-Cas9 Vectors  
Cas9 Protein
EGFP-labeled Cas9 ProteinMODIFYING CELL LINES

• Stable KO, KI, and genome editing of
  somatic cells


• Transgenic cell line generation


• Cell-based disease models

Cells that are transfectableCas9 Plasmids
Cas9 Protein
EGFP-labeled Cas9 ProteinDifficult-to-transfect cell lines:

• Primary cells


• Hematopoietic cells


• Stem cells

AAV-Cas9 Vectors 
Lenti Cas9 SystemsSCREENING

• Genome-wide surveys


• gRNA library screens


• Functional screens

All cell types requiring stable Cas9 overexpressionLenti Cas9 Systems
AAVS1 Safe Harbor Site Cas9
Gene Knock-in System
Cas9 Protein
EGFP-labeled Cas9 ProteinPRE-CLINICAL APPLICATIONS

• Off-target events are of highest concern

All cell types and applicationsCas9 Nickase, available in all delivery formats
Cas9 Protein
EGFP-labeled Cas9 ProteinSIMULTANEOUS ENGINEERING OF MULTIPLE MUTATIONSAll cell types and applicationsMultiplex gRNA cloning kit, compatible with all Cas9 delivery options SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应