Creating target-specific EVs just got a whole lot easier

The XStamp™ Pro Streptavidin EV Targeting Kit gives you virtually unlimited target specificity options. Just decorate the outside of your extracellular vesicles (EVs) with XStamp Pro Streptavidin and then attach the biotinylated targeting moiety partner of your choice, such as a biotinylated ScFv antibody. (See Table 1 for the pre-made XStamp Pro options). Save time and simplify your EV engineering workflow with XStamp Pro.

While conventional methods for creating target-specific EVs are highly robust (see our XStamp Cloning and Expression Lentivector, Cat# XSTP710PA-1), they involve cloning and manipulation of the producer cells which can be challenging, especially if the cells are hard to transfect. To streamline this process and help researchers more quickly and easily create EVs that preferentially localize to a specific target, SBI turned to an exciting new technology, XStamp Pro, which works on already purified EVs—no cloning or transfection required. Simply incubate purified EVs with the XStamp Pro reagent for one-hour and then isolate your target-specific EVs.

• Speed production of target-specific EVs with the next generation of XStamp technology
• Streamline engineering by working directly with purified EVs—no expression vector or transfection required
• Choose what you target and how you target by using any biotinylated moiety
• Save hands-on time with a simple workflow—just incubate EVs with XStamp Pro Streptavidin for one hour, add a biotinylated targeting moiety, and isolate target-specific EVs
• Easily generate multiple targeting variants for quick screening studies
• Compatible with any EV loading protocol

Each XStamp Pro Streptavidin Kit comes with enough XStamp Pro Streptavidin for 10 reactions* and ExoQuick®-TC for EV isolation. Once the reaction is complete and your streptavidin-coated EVs are isolated, they are ready for addition of the biotinylated moiety, cargo-loading, and cargo delivery.

For even faster workflows, you can use Exo-Fect siRNA/miRNA EV Transfection Reagent (Cat.# EXFT200A-1) or the original Exo-Fect Reagent (Cat.# EXFT10A-1 /20A-1) to load cargo into isolated EVs before adding the targeting specificity with XStamp Pro.

Cat. #	Product	Specificity
XSTP900A-1	XStamp Pro Streptavidin	Any - determined by user-supplied biotinylated moiety
XSTP905A-1	XStamp Pro anti-HIV	HIV-infected cells
XSTP910A-1	XStamp Pro anti-CD16	CD16-expressing cells
XSTP915A-1	XStamp Pro anti-PD-1	PD-1-epxressing cells

*One reaction can label up to 300 µg protein equivalent of EVs. SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应