MonitorexosomedynamicswiththepCT-CD81-GFP(pCMV)ExosomeCyto-Tracer(alsoavailableasaCD81-RFPfusion,pCT-CD81-RFP).Providedaseitherlentivectororpre-packagedvirus,thisCyto-TracerfusesGFPtothetetraspaninCD81,whichthengetspackagedintoexosomesproducedbythetransfectedcells.GreatforcreatingstablecelllinesthatproduceGFP-labeledexosomesaswellaslabelingexosomesfromprimarycells,tumorcells,stemcells,andmore.
- Stablelentivector-basedsystem
- Idealforco-localizationstudies
- Monitorexosomedynamicsandfunctionalstudiesinrealtime
- Labelexosomesfromprimarycells,tumorcells,stemcells,andmore
Choosetheexosomequantitationmethodthat’sbestforyourstudies
| Promoter(Cat.#) | Cyto-Tracer | TargetLocation | PeptideTag |
|---|
CMV(CYTO100-PA-1/CYTO100-VA-1)
MSCV(CYTO100-PB-1/CYTO100-VB-1) | pCT-Mem-GFP | PlasmaMembrane | Neuromodulin |
CMV(CYTO101-PA-1/CYTO101-VA-1)
MSCV(CYTO101-PB-1/CYTO101-VB-1) | pCT-InnerMem-GFP | InnerLeafletofMembrane | Farnesylationsignal |
CMV(CYTO102-PA-1/CYTO102-VA-1)
MSCV(CYTO102-PB-1/CYTO102-VB-1) | pCT-Mito-GFP | Mitochondria | CytochromeCoxidaseVIII |
| CMV(CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | EndoplasmicReticulum | ERtargetingconsensus |
| CMV(CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | GolgiApparatus | Galactosyltrasferase |
| CMV(CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretoryconsensuspeptide |
| CMV(CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | CathepsinB |
| CMV(CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
| CMV(CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
CMV(CYTO108-PA-1/CYTO108-VA-1)
MSCV(CYTO108-PB-1/CYTO108-VB-1) | pCT-Pero-GFP | Peroxisome | Peroxisomalconsensussignal |
| CMV(CYTO120-PA-1/CYTO120-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
| CMV(CYTO120R-PA-1/CYTO120R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
| CMV(CYTO109-PA-1/CYTO109-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63Tetraspanin |
| CMV(CYTO109R-PA-1/CYTO109R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63Tetraspanin |
| CMV(CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9Tetraspanin |
| CMV(CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9Tetraspanin |
| CMV(CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81Tetraspanin |
| CMV(CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81Tetraspanin |
CMV(CYTO110-PA-1/CYTO110-VA-1)
MSCV(CYTO110-PB-1/CYTO110-VB-1) | pCT-Actin-GFP | Cytoskeleton | β-actin |
CMV(CYTO111-PA-1/CYTO111-VA-1)
MSCV(CYTO111-PB-1/CYTO111-VB-1) | pCT-Tublin-GFP | Cytoskeleton | α-tublin |
| CMV(CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
| CMV(CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubuleassociatedprotein4(MAP4) |
CMV(CYTO113-PA-1/CYTO113-VA-1)
MSCV(CYTO113-PB-1/CYTO113-VB-1) | pCT-H2B-GFP | Nucleus | H2B |
| CMV(CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase3/7activation | Cyclicinactiveluciferase |
| CMV(CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | CytosoltoMitochondria | BAX |
| CMV(CYTO116-PA-1/CYTO116-VA-1) | pCT-Catenin-GFP | CytosoltoNucleus/CellMembrane | β-Cateninactivation |
| CMV(CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | DendriteMembranes | PSD-95 |
CMV(CYTO118-PA-1/CYTO118-VA-1)
MSCV(CYTO118-PB-1/CYTO118-VB-1) | pCT-Cyto-GFP | CytosolicCompartment | GFP-Untagged |
| CMV(CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | CytosolicCompartment | RFP-Untagged |
| MSCV(CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFPandLuciferaseControl | GFPandLuciferase(Untagged) |
SeesomeofourexosomeCyto-Tracersinaction
Thefollowingfigureandvideosarefrom:
GarciaNA,etal.GlucoseStarvationinCardiomyocytesEnhancesExosomeSecretionandPromotesAngiogenesisinEndothelialCells.PLoSONE.2015.10(9).PMCID:PMC4578916.
Figure4fromGarcia,etal.ExosometransferfromCMstoECs.(A)H9c2transfectedwithpCT-CD63-GFP.FACSanalysisof90%GFPpositivecells.(B)Representativeimagesfromconfocaltime-lapsemicroscopyofmouseECs(ACTB-DsRed)(EC;red)co-culturedwithH9C2-CD63-GFPcells(green),previouslyculturedfor24hin+/-Stmedium.ExosometransferfromH9C2CMstoECcanbeobserved(S1andS2Movies,below).WhitearrowsshowCD63-GFPstructuresinsideECs(C)RepresentativeimmunostainingofH9C2-CD63-GFPandHUVECco-cultures;anti-GFP(green)andanti-CD31(red).TheimagesillustrateGFPfluorescencefromCD63-GFPexosomesinCD31-positivecells(red)after24hincubationin+/-Stmedium.WhitearrowsshowCD63-GFPstructuresinsideECs.
Labeledexosomes.(Toppanels)CD63-GFPCyto-Tracerstransfectedintoahumanfibrosarcomacellline.(Bottompanels)CD9-GFPandCD9-RFPCyto-Tracersco-transfectedintoHEK293cells.
Citations:
Braganza,A,etal.(2017)UBE3BIsaCalmodulin-regulated,Mitochondrion-associatedE3UbiquitinLigase.J.Biol.Chem..2017Feb10;292(6):2470-2484.PMID:28003368
Gonzalez-King,H,etal.(2017)HypoxiaInduc
IBLeFactor-1αPotentiatesJagged1-MediatedAngiogenesisbyMesenchymalStemCell-DerivedExosomes.
StemCells.2017Jul1;35(7):1747-1759.PMID:28376567
Jawhari,S,etal.(2017)AutophagyandTrkC/NT-3signalingjoinedforcesboostthehypoxicglioblastomacellsurvival.Carcinogenesis.2017Jun1;38(6):592-603.PMID:28402394
Soares,VYR.(2017)OpapeldasvesículasextracelularesnafisiopatologiadaperdaauditivaocasionadapeloSchwannomavestibular.Thesis.;.Link:Thesis
Yoon,C,etal.(2016)DeliveryofmiR-155toretinalpigmentepithelialcellsmediatedbyBurkitt’slymphomaexosomes.TumourBiol..2016Jan1;37(1):313-21.PMID:26211004
Jawhari,S.(2015)Etudedessignalisationsautophagiqueetneurotrophiquedansdeslignéesdeglioblastomehumainactivéeslorsdel’hypoxie.Thesis.;.Link:Thesis
LoCicero,A,etal.(2015)Exosomesreleasedbykeratinocytesmodulatemelanocytepigmentation.NatCommun.2015Jun24;6:7506.PMID:26103923
VARLET,P,PU-PH,P&DJAVAHERI-MERGNY,M.(2015)SOHAJAWHARI.Thesis.;.Link:Thesis
Liang,Y.(2015)Exosome/MicrovesicleCargoContentsRecruitmentinaGlycosylation-dependentPathway.Thesis.;.Link:Thesis
Sato,Y,etal.(2014)MTCL1crosslinksandst
ABIlizesnon-centrosomalmicrotubulesontheGolgimembrane.
NatCommun.2014Nov4;5:5266.PMID:25366663
Liang,Y,etal.(2014)ComplexN-linkedglycansserveasadeterminantforexosome/microvesiclecargorecruitment.J.Biol.Chem..2014Nov21;289(47):32526-37.PMID:25261472
Fang,Q,etal.(2014)HSP90regulatesDNArepairviatheinteractionbetweenXRCC1andDNApolymeraseβ.NatCommun.2014Nov26;5:5513.PMID:25423885
SBI ExoQuick PLUS 系列
高纯度 Exosome 分离—满足敏感下游实验
ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。
产品特色
Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白
Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留
Powerful — 最多可 3 倍提高 biomarker 检测效率
Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可
试剂盒组分
ExoQuick PLUS 试剂盒
ExoQuick Reagent
2 mL
ExoQuick PLUS Microsphere Beads
10 units
Resuspension Buffer
15 mL
注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。
ExoQuick-TC PLUS 试剂盒
ExoQuick-TC Reagent
10 mL
ExoQuick PLUS Microsphere beads
10 units
Resuspension Buffer
15 mL
注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。
产品信息
货号
产品描述
规格
说明
PM-EQPL10A-1
ExoQuick PLUS Exosome Purification Kit
10 rxns
以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。
PM-EQPL10TC-1
ExoQuick-TC PLUS Exosome Purification Kit
10 rxns
以 5mL 细胞培养上清为 1 次反应
