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Irvine Scientific/91166 BalanCD HEK 293 Feed/91166/1 Ea

价格
面议
货号:91166
浏览量:127
品牌:Irvine
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商品描述

BalanCDHEK293®FeedisformulatedtosignificantlyincreaseproteinyieldsinsUSPension-adaptedHEK293celllinespost-transfection.

  • Increaseviablecelldensityandculturedurationtomaximizeyield
  • UsethesameFeedforawiderangeofHEK293celllines
  • Fulfillregulatoryrequirements

•Chemically-defined,animalcomponent-freeformula
•DrugMasterFile(DMF)submittedtoUSFDA

Two-foldincreaseintitercomparedtoothercommercially-availablemedia


 

HEK293-6Ecellswereculturedinduplicate125mLErlenmeyerflaskswitha30mLworkingvolume.Cellswereseededat3x105cells/mLinBalanCDHEK293medium.PEI-mediatedtransfectionwasperformedonday3toproduceabiosimilarantibody.BalanCDHEK293mediumwassupplementedwitha5%v/vadditionofBalanCDHEK293Feedondays1-4post-transfection. Proteintiterwasmeasuredondays7,9,and11post-transfection.CulturesterminatedwhenviABIlitydroppedbelow70%,(datanotshown).Datashownrepresentstheaverageoftwoduplicatecultures.
 
Storage

2-8°C

ShelfLife

12monthsfromthedateofmanufacture(DOM)

CHANG产品组合中的每种专门培养基均经过优化,可在细胞遗传学样品中产生优异的生长和形态。它们辅以一系列经典培养基,盐,缓冲液和试剂。我们的媒体是cGMP制造的,经过性能测试,让您高枕无忧。依靠CHANG媒体进行成功的分析30多年来,FUJIFILM Irvine Scientific在提供针对细胞遗传学实验室量身定制的全面产品方面一直处于世界领先地位。CHANG是专门为细胞遗传学设计的原始培养基,数十年来一直是金标准。我们当前的CHANG投资组合以现代公式为基础。每种细胞或组织特异性培养基均经过优化,可在外周血,骨髓,羊水,绒毛膜(CVS)和受孕产物(POC)样品中产生优异的生长和形态。