Our anti-TREM2 chicken primary antibody from AvesLabs is manufactured in-house. It detectsTREM2, and is purified. It is provided in liquid form.

Volume: 100µL (TREM2-0020) or 500µL (TREM2-0100)

Concentration: 0.2mg/mL

Clonality: Polyclonal

Form: Purified

Host Species: Chicken

Antibody Isotype: IgY

Species Reactivity: Human, Mouse, Rat

Applications: ICC, WB

Immunogen: Purified recombinant extracellular domain of human TREM2 (Uniprot Q9NZC2) expressed in E.Coli

Protein Name / Synonyms: Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2

Target Description: Triggering receptor expressed on myeloid cells 2, TREM2, is a type I transmembrane innate immune receptor predominantly expressed by microglia in the central nervous system and is genetically linked to the risk for Alzheimer’s disease (Schmid, C.D., et al., 2002). TREM2 has been shown to be a specific marker for alternatively activated microglia known as M2 cells (Cherry, J.D. et al., 2014). TREM2 binds to its adaptor, DNAX-activating protein (DAP12) on the surface of microglia to enact innate immune responses and downstream cellular responses or signaling pathways (Jay,T.R, et al., 2017). TREM2 has important roles in microglial phagocytosis of apoptotic neurons, damaged myelin, and amyloid plaques (Gratuze, M., et al., 2018). In early brain development, TREM2 is essential for synaptic pruning (Filipello, F. et al., 2018).

Gene ID: TREM2

Physical State: Liquid

Conjugate: Unconjugated

Production Notes: Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Validation and Application Notes

Molecular Weight: 36 kDa

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。