Details

GoatswereimmunizedwithpurifiedchickenIgY(wholeprotein)emulsifiedinFreund’sadjuvant.Aftermultipleinjections,goatswerebledandserumcollected.Antibodieswereisolatedusingaffinitychromatographyandconjugatedtoalkalinephosphatase.

Volume:1mL

Concentration:0.1mg/mL

Clonality:Polyclonal

Form:AffinityPurified

HostSpecies:Goat

SpeciesReactivity:Chicken

Immunogen:IgY

PhysicalState:Liquid

Conjugate:AP

Buffer:HEPES(50mM,pH7.0),NaCl(0.1M),bovineserumalbumin(0.2%,w/v),MgCl2(1.0mM),ZnCl2(0.1mM),sodiumazide(0.02%,w/v)(asanantimicrobialagent).

Specificity:Doesnotcross-reactwithmammalianIgGs

QualityControl

Antibodieswereanalyzedusingimmunoelectrophoresis(seebelow)andasandwichELISA.Inthislattertest,a1:800dilutionofGGHL-30PH-2wasfoundtogenerateanO.Dof1.0ina20minutereactionwithp-nitrophenylphosphateasthesubstrate.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Forlongerstorageperiods,dilutetheantibodieswithsterileglycerolandstoreat-20°C.Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

↑backtotop

鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。