Details

Chickenswereimmunizedwithasyntheticpeptide/keyholelimpethemocyanin(KLH)conjugate.ThepeptidecorrespondedtoaregionoftheCNPasegeneproductsharedbetweenthehuman(NP_149124,NCBI)andmouse(P16330,NCBI)sequences.Afterrepeatedinjections,immuneeggswerecollected,theIgYfractionswerepurifiedfromtheyolks,andthenaffinity-purifiedusingapeptidecolumn.Theconcentrationsoftheeluateswerethenadjustedto100µg/mL,andthepreparationwasfilter-sterilized.

Volume:1000µL

Concentration:100µg/mL

Clonality:Polyclonal

HostSpecies:Chicken

SpeciesReactivity:Human,Mouse,Rat

Applications:ICC,IHC,WB

ProteinName/Synonyms:2",3"-cyclic-nucleotide3"-phosphodiesterase,CNP,CNPase,EC3.1.4.37

GeneID:CNP

AntibodyRegistryID(RRID):AB_2313538

PhysicalState:Liquid

ValidationandApplicationNotes

MolecularWeight:47kDa

WesternBlotDilutionRange:1:500-1:1000

IHCDilutionRange:1:500-1:1000

QualityControl

Qualityassuranceanalysiswasperformedusingimmunohistochemistry(atadilutionof1:2000)usingfluorescein-labeledgoatanti-chickenIgY(1:500dilution,AvesLabsCat.#F-1005)asthesecondaryreagent.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.Note,however,thateachtimeanantibodypreparationisfrozen,abouthalfofitsbindingactivityislost.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

↑backtotop

鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。