Details

Chickens were immunized with a synthetic peptide containing the human c-myc epitope tag – EQKLISEEDL – conjugated to keyhole limpet hemocyanin (KLH).The sequence of this peptide corresponds to residues #425-434 of the human c-myc proto-oncogene protein (NCBI NP_002458.2).After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.These IgY fractions were then affinity-purified using an agarose matrix to which the cognate peptide was attached.Eluates of this column were dialyzed against PBS, and then conjugated to fluorescein using its isothiocyanate form (FITC).Fluorescein-labeled antibody was separated from free FITC using gel exclusion chromatography (Sephadex G-50), and the protein concentration was adjusted to 0.1 mg/mL in PBS with 0.02% sodium azide.Finally, the preparation was filter-sterilized.

Volume: 1 mL

Clonality: Polyclonal

Host Species: Chicken

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。