| Chemical Name: | 3-(6-methylpyridin-2-yl)-N-phenyl-4-(quinolin-4-yl)-1H-pyrazole-1-carbothioamide |
| Molecular Weight: | 421.52 |
| Formula: | C25H19N5S |
| Purity: | ≥ 98% |
| CAS#: | 909910-43-6 |
| Solubility: | DMSO up to 100 mM |
| Storage: | Powder:4oC 1 year DMSO:4oC3 month-20oC 1 year |
A83-01 is a potent and selective TGF-β pathway inhibitor. It inhibits ALK5 (IC50~12 nM), ALK4 (IC50~45 nM) and ALK7 (IC50~7.5nM). It only has weak inhibitory activity on ALK1, 2, 3, and 6. It blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. A83-01 has been used to enhance iPSC reprogramming, induce neural differentiation of human pluripotent stem cells, and promote naïve state of pluripotent stem cells.
How to Use:
In vitro: A83-01 is typically used at 10 µM as final concentration in cell culture.
In vivo: A83-01 was orally dosed to mice at 100-200 mg/kg once per day or intraperitoneally dosed to mice at 10-100 mg/kg once per day.
Reference:
- 1. Tojo M, et al. The ALK-5 inhibitor A-83-01 inhibits smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta. (2005) Cancer Science 96 (11), 791-800.
- 2. Li W, et al. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. (2009) Cell Stem Cell 4(1), 16-19.
- 3. Yuan X, et al. Combined Chemical Treatment Enables Oct4-Induced Reprogramming from Mouse Embryonic Fibroblasts. (2011) Stem Cells 29(3), 549–553.
- 4. Klincumhom N, et al. Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells. (2014) Neurosci Lett. 578:1-6.
A83-01_spec.pdf
A83-01_MSDS.pdfProducts are for research use only. Not for human use.
